-
Something wrong with this record ?
In vitro activity of antibiotics potentially effective against difficult-to-treat strains of Gram-negative rods: retrospective study
G. Kroneislová, J. Závora, VG. Adámková, A. Rýdlová, V. Adámková
Language English Country England, Great Britain
Document type Journal Article
Grant support
MH CZ-DRO-VFN00064165
Ministerstvo Zdravotnictví Ceské Republiky
MH CZ-DRO-VFN00064165
Ministerstvo Zdravotnictví Ceské Republiky
MH CZ-DRO-VFN00064165
Ministerstvo Zdravotnictví Ceské Republiky
NLK
Directory of Open Access Journals
from 2011
Free Medical Journals
from 2011
Nature Open Access
from 2011-12-01
PubMed Central
from 2011
Europe PubMed Central
from 2011
ProQuest Central
from 2011-01-01
Open Access Digital Library
from 2011-01-01
Open Access Digital Library
from 2011-01-01
Health & Medicine (ProQuest)
from 2011-01-01
ROAD: Directory of Open Access Scholarly Resources
from 2011
Springer Nature OA/Free Journals
from 2011-12-01
- MeSH
- Anti-Bacterial Agents * pharmacology therapeutic use MeSH
- Azabicyclo Compounds * MeSH
- Aztreonam MeSH
- Cephalosporins * MeSH
- Cefiderocol MeSH
- Gram-Negative Bacteria MeSH
- Colistin pharmacology MeSH
- Humans MeSH
- Microbial Sensitivity Tests MeSH
- Pseudomonas aeruginosa MeSH
- Retrospective Studies MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
Bacterial resistance surveillance is one of the main outputs of microbiological laboratories and its results are important part of antimicrobial stewardship (AMS). In this study, the susceptibility of specific bacteria to selected antimicrobial agents was tested. The susceptibility of 90 unique isolates of pathogens of critical priority obtained from clinically valid samples of ICU patients in 2017-2021 was tested. 50% of these fulfilled difficult-to-treat resistance (DTR) criteria and 50% were susceptible to all antibiotics included in the definition. 10 Enterobacterales strains met DTR criteria, and 2 (20%) were resistant to colistin (COL), 2 (20%) to cefiderocol (FCR), 7 (70%) to imipenem/cilastatin/relebactam (I/R), 3 (30%) to ceftazidime/avibactam (CAT) and 5 (50%) to fosfomycin (FOS). For Enterobacterales we also tested aztreonam/avibactam (AZA) for which there are no breakpoints yet. The highest MIC of AZA observed was 1 mg/l, MIC range in the susceptible cohort was 0.032-0.064 mg/l and in the DTR cohort (incl. class B beta-lactamase producers) it was 0.064-1 mg/l. Two (13.3%) isolates of Pseudomonas aeruginosa (15 DTR strains) were resistant to COL, 1 (6.7%) to FCR, 13 (86.7%) to I/R, 5 (33.3%) to CAT, and 5 (33.3%) to ceftolozane/tazobactam. All isolates of Acinetobacter baumannii with DTR were susceptible to COL and FCR, and at the same time resistant to I/R and ampicillin/sulbactam. New antimicrobial agents are not 100% effective against DTR. Therefore, it is necessary to perform susceptibility testing of these antibiotics, use the data for surveillance (including local surveillance) and conform to AMS standards.
Department of Infectious Disease Faculty of Medicine Imperial College London London UK
Department of Plant Science Cambridge University Cambridge UK
References provided by Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc24014345
- 003
- CZ-PrNML
- 005
- 20240905134218.0
- 007
- ta
- 008
- 240725s2024 enk f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1038/s41598-024-59036-0 $2 doi
- 035 __
- $a (PubMed)38594467
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a enk
- 100 1_
- $a Kroneislová, Gabriela $u Department of Clinical Microbiology and ATB Centre, Institute of Medical Biochemistry and Laboratory Diagnostic, First Faculty of Medicine, Charles University and General University Hospital, Ke Karlovu 2, Prague, 12808, Czech Republic. gabriela.kroneislova@vfn.cz $u Department of Biochemistry and Microbiology, Faculty of Food and Biochemical Technology, University of Chemistry and Technology, Prague, Czech Republic. gabriela.kroneislova@vfn.cz
- 245 10
- $a In vitro activity of antibiotics potentially effective against difficult-to-treat strains of Gram-negative rods: retrospective study / $c G. Kroneislová, J. Závora, VG. Adámková, A. Rýdlová, V. Adámková
- 520 9_
- $a Bacterial resistance surveillance is one of the main outputs of microbiological laboratories and its results are important part of antimicrobial stewardship (AMS). In this study, the susceptibility of specific bacteria to selected antimicrobial agents was tested. The susceptibility of 90 unique isolates of pathogens of critical priority obtained from clinically valid samples of ICU patients in 2017-2021 was tested. 50% of these fulfilled difficult-to-treat resistance (DTR) criteria and 50% were susceptible to all antibiotics included in the definition. 10 Enterobacterales strains met DTR criteria, and 2 (20%) were resistant to colistin (COL), 2 (20%) to cefiderocol (FCR), 7 (70%) to imipenem/cilastatin/relebactam (I/R), 3 (30%) to ceftazidime/avibactam (CAT) and 5 (50%) to fosfomycin (FOS). For Enterobacterales we also tested aztreonam/avibactam (AZA) for which there are no breakpoints yet. The highest MIC of AZA observed was 1 mg/l, MIC range in the susceptible cohort was 0.032-0.064 mg/l and in the DTR cohort (incl. class B beta-lactamase producers) it was 0.064-1 mg/l. Two (13.3%) isolates of Pseudomonas aeruginosa (15 DTR strains) were resistant to COL, 1 (6.7%) to FCR, 13 (86.7%) to I/R, 5 (33.3%) to CAT, and 5 (33.3%) to ceftolozane/tazobactam. All isolates of Acinetobacter baumannii with DTR were susceptible to COL and FCR, and at the same time resistant to I/R and ampicillin/sulbactam. New antimicrobial agents are not 100% effective against DTR. Therefore, it is necessary to perform susceptibility testing of these antibiotics, use the data for surveillance (including local surveillance) and conform to AMS standards.
- 650 _2
- $a lidé $7 D006801
- 650 12
- $a antibakteriální látky $x farmakologie $x terapeutické užití $7 D000900
- 650 _2
- $a retrospektivní studie $7 D012189
- 650 12
- $a cefalosporiny $7 D002511
- 650 _2
- $a aztreonam $7 D001398
- 650 _2
- $a cefiderokol $7 D000097602
- 650 _2
- $a gramnegativní bakterie $7 D006090
- 650 _2
- $a kolistin $x farmakologie $7 D003091
- 650 _2
- $a mikrobiální testy citlivosti $7 D008826
- 650 _2
- $a Pseudomonas aeruginosa $7 D011550
- 650 12
- $a azabicyklické sloučeniny $7 D053961
- 655 _2
- $a časopisecké články $7 D016428
- 700 1_
- $a Závora, Jan $u Department of Clinical Microbiology and ATB Centre, Institute of Medical Biochemistry and Laboratory Diagnostic, First Faculty of Medicine, Charles University and General University Hospital, Ke Karlovu 2, Prague, 12808, Czech Republic $u Department of Microbiology, Faculty of Medicine and Dentistry, Palacký University-Olomouc, Olomouc, Czech Republic
- 700 1_
- $a Adámková, Vanda Gabriela $u Department of Plant Science, Cambridge University, Cambridge, UK
- 700 1_
- $a Rýdlová, Anna $u Department of Infectious Disease, Faculty of Medicine, Imperial College London, London, UK
- 700 1_
- $a Adámková, Václava $u Department of Clinical Microbiology and ATB Centre, Institute of Medical Biochemistry and Laboratory Diagnostic, First Faculty of Medicine, Charles University and General University Hospital, Ke Karlovu 2, Prague, 12808, Czech Republic
- 773 0_
- $w MED00182195 $t Scientific reports $x 2045-2322 $g Roč. 14, č. 1 (2024), s. 8310
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/38594467 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y - $z 0
- 990 __
- $a 20240725 $b ABA008
- 991 __
- $a 20240905134212 $b ABA008
- 999 __
- $a ok $b bmc $g 2143865 $s 1226211
- BAS __
- $a 3
- BAS __
- $a PreBMC-MEDLINE
- BMC __
- $a 2024 $b 14 $c 1 $d 8310 $e 20240409 $i 2045-2322 $m Scientific reports $n Sci Rep $x MED00182195
- GRA __
- $a MH CZ-DRO-VFN00064165 $p Ministerstvo Zdravotnictví Ceské Republiky
- GRA __
- $a MH CZ-DRO-VFN00064165 $p Ministerstvo Zdravotnictví Ceské Republiky
- GRA __
- $a MH CZ-DRO-VFN00064165 $p Ministerstvo Zdravotnictví Ceské Republiky
- LZP __
- $a Pubmed-20240725
