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Cultivation driven transcriptomic changes in the wild-type and mutant strains of Rhodospirillum rubrum

K. Jureckova, M. Nykrynova, E. Slaninova, H. Fleuriot-Blitman, V. Amstutz, K. Hermankova, M. Bezdicek, K. Mrazova, K. Hrubanova, M. Zinn, S. Obruca, K. Sedlar

. 2024 ; 23 (-) : 2681-2694. [pub] 20240620

Status neindexováno Jazyk angličtina Země Nizozemsko

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc24017721

Purple photosynthetic bacteria (PPB) are versatile microorganisms capable of producing various value-added chemicals, e.g., biopolymers and biofuels. They employ diverse metabolic pathways, allowing them to adapt to various growth conditions and even extreme environments. Thus, they are ideal organisms for the Next Generation Industrial Biotechnology concept of reducing the risk of contamination by using naturally robust extremophiles. Unfortunately, the potential of PPB for use in biotechnology is hampered by missing knowledge on regulations of their metabolism. Although Rhodospirillum rubrum represents a model purple bacterium studied for polyhydroxyalkanoate and hydrogen production, light/chemical energy conversion, and nitrogen fixation, little is known regarding the regulation of its metabolism at the transcriptomic level. Using RNA sequencing, we compared gene expression during the cultivation utilizing fructose and acetate as substrates in case of the wild-type strain R. rubrum DSM 467T and its knock-out mutant strain that is missing two polyhydroxyalkanoate synthases PhaC1 and PhaC2. During this first genome-wide expression study of R. rubrum, we were able to characterize cultivation-driven transcriptomic changes and to annotate non-coding elements as small RNAs.

Citace poskytuje Crossref.org

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$a Nykrynova, Marketa $u Department of Biomedical Engineering, Faculty of Electrical Engineering and Communication, Brno University of Technology, Brno, Czech Republic
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$a Slaninova, Eva $u Department of Food Chemistry and Biotechnology, Faculty of Chemistry, Brno University of Technology, Brno, Czech Republic
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$a Fleuriot-Blitman, Hugo $u Institute of Life Technologies, University of Applied Sciences and Arts Western Switzerland Valais-Wallis (HES-SO Valais-Wallis), Sion, Switzerland
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$a Amstutz, Véronique $u Institute of Life Technologies, University of Applied Sciences and Arts Western Switzerland Valais-Wallis (HES-SO Valais-Wallis), Sion, Switzerland
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$a Mrazova, Katerina $u Department of Food Chemistry and Biotechnology, Faculty of Chemistry, Brno University of Technology, Brno, Czech Republic $u Institute of Scientific Instruments of the Czech Academy of Sciences, v.v.i., Brno, Czech Republic
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$a Obruca, Stanislav $u Department of Food Chemistry and Biotechnology, Faculty of Chemistry, Brno University of Technology, Brno, Czech Republic
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