• Je něco špatně v tomto záznamu ?

Proteomic exploration of potential blood biomarkers in haemophilic arthropathy

N. Kalebota, R. Novak, S. Hrkač, P. Perić, G. Salai, M. Močibob, M. Pranjić, Z. Zdráhal, V. Pustka, NL. Žerjavić, M. Milošević, M. Vodanović, SZ. Šalek, L. Grgurević

. 2024 ; 7 (9) : e70046. [pub] 20240925

Status neindexováno Jazyk angličtina Země Spojené státy americké

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc24017761

BACKGROUND AND AIMS: The pathophysiology of haemophilic arthropathy (HA) is complex and largely undefined. Proteomic analyses provide insights into the intricate mechanisms of the HA.Our study aimed to identify differentially expressed proteins in relation to the severity of HA, explore their pathophysiological roles, and evaluate their potential as HA biomarkers. METHODS: Our cross-sectional observational study encompassed 30 HA patients and 15 healthy subjects. Plasma samples were pooled into three groups of 15 samples from those with severe haemophilic arthropathy (sHA), mild haemophilic arthropathy (mHA) and healthy controls. Proteomic analysis was performed using liquid chromatography-mass spectrometry. The severity of HA was assessed using the World Federation of Haemophilia Physical Examination Score and ultrasonography following the Haemophilia Early Arthropathy Detection with Ultrasound (HEAD-US) guidelines. RESULTS: A total of 788 proteins were identified, with 97% of the uniquely identified proteins being expressed in all analysed groups. We identified several up and downregulated proteins across the groups that were mainly related to inflammatory and immunity-modulating processes, as well as joint degeneration. We highlighted ten proteins relevant for the development of HA: cathepsin G, endoplasmic reticulum aminopeptidase 2, S100-A9, insulin-like growth factor I, apolipoprotein (a), osteopontin, pregnancy zone protein, cartilage oligomeric matrix protein, CD44, and cadherin-related family member 2. CONCLUSION: Our analysis identified several proteins that shed further light on the distinctive pathogenesis of HA and could serve for biomarker research. However, these results need to be validated on a larger patient group.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc24017761
003      
CZ-PrNML
005      
20241016082013.0
007      
ta
008      
241008s2024 xxu f 000 0|eng||
009      
AR
024    7_
$a 10.1002/hsr2.70046 $2 doi
035    __
$a (PubMed)39323462
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xxu
100    1_
$a Kalebota, Nataša $u Department of Rheumatology and Rehabilitation University Hospital Centre Zagreb Zagreb Croatia
245    10
$a Proteomic exploration of potential blood biomarkers in haemophilic arthropathy / $c N. Kalebota, R. Novak, S. Hrkač, P. Perić, G. Salai, M. Močibob, M. Pranjić, Z. Zdráhal, V. Pustka, NL. Žerjavić, M. Milošević, M. Vodanović, SZ. Šalek, L. Grgurević
520    9_
$a BACKGROUND AND AIMS: The pathophysiology of haemophilic arthropathy (HA) is complex and largely undefined. Proteomic analyses provide insights into the intricate mechanisms of the HA.Our study aimed to identify differentially expressed proteins in relation to the severity of HA, explore their pathophysiological roles, and evaluate their potential as HA biomarkers. METHODS: Our cross-sectional observational study encompassed 30 HA patients and 15 healthy subjects. Plasma samples were pooled into three groups of 15 samples from those with severe haemophilic arthropathy (sHA), mild haemophilic arthropathy (mHA) and healthy controls. Proteomic analysis was performed using liquid chromatography-mass spectrometry. The severity of HA was assessed using the World Federation of Haemophilia Physical Examination Score and ultrasonography following the Haemophilia Early Arthropathy Detection with Ultrasound (HEAD-US) guidelines. RESULTS: A total of 788 proteins were identified, with 97% of the uniquely identified proteins being expressed in all analysed groups. We identified several up and downregulated proteins across the groups that were mainly related to inflammatory and immunity-modulating processes, as well as joint degeneration. We highlighted ten proteins relevant for the development of HA: cathepsin G, endoplasmic reticulum aminopeptidase 2, S100-A9, insulin-like growth factor I, apolipoprotein (a), osteopontin, pregnancy zone protein, cartilage oligomeric matrix protein, CD44, and cadherin-related family member 2. CONCLUSION: Our analysis identified several proteins that shed further light on the distinctive pathogenesis of HA and could serve for biomarker research. However, these results need to be validated on a larger patient group.
590    __
$a NEINDEXOVÁNO
655    _2
$a časopisecké články $7 D016428
700    1_
$a Novak, Ruđer $u Centre for Translational and Clinical Research, Department of Proteomics University of Zagreb, School of Medicine Zagreb Croatia $u BIMIS - Biomedical Research Centre Šalata University of Zagreb School of Medicine Zagreb Croatia
700    1_
$a Hrkač, Stela $u Department of Clinical Immunology Allergology and Rheumatology, University Hospital Dubrava Zagreb Croatia
700    1_
$a Perić, Porin $u Department of Rheumatology and Rehabilitation University Hospital Centre Zagreb Zagreb Croatia $u University of Zagreb, School of Medicine Zagreb Croatia
700    1_
$a Salai, Grgur $u Department of Pulmonology University Hospital Dubrava Zagreb Croatia
700    1_
$a Močibob, Marko $u Department of Chemistry University of Zagreb, Faculty of Science Zagreb Croatia
700    1_
$a Pranjić, Marija $u Department of Chemistry University of Zagreb, Faculty of Science Zagreb Croatia
700    1_
$a Zdráhal, Zbyněk $u Central European Institute of Technology Masaryk University, Kamenice 5 Brno Czech Republic
700    1_
$a Pustka, Václav $u Central European Institute of Technology Masaryk University, Kamenice 5 Brno Czech Republic
700    1_
$a Žerjavić, Nadica Laktašić $u Department of Rheumatology and Rehabilitation University Hospital Centre Zagreb Zagreb Croatia $u University of Zagreb, School of Medicine Zagreb Croatia
700    1_
$a Milošević, Milan $u Andrija Štampar School of Public Health University of Zagreb, School of Medicine Zagreb Croatia
700    1_
$a Vodanović, Marijo $u Division of Hematology, Department of Internal Medicine University Hospital Centre Zagreb Zagreb Croatia $u University of Applied Health Sciences Zagreb Croatia
700    1_
$a Šalek, Silva Zupančić $u Department of Hematology Clinical Hospital "Sv. Duh" Zagreb Croatia
700    1_
$a Grgurević, Lovorka $u Centre for Translational and Clinical Research, Department of Proteomics University of Zagreb, School of Medicine Zagreb Croatia $u BIMIS - Biomedical Research Centre Šalata University of Zagreb School of Medicine Zagreb Croatia $u Department of Anatomy, "Drago Perović" University of Zagreb, School of Medicine Zagreb Croatia $1 https://orcid.org/0000000298143178
773    0_
$w MED00207111 $t Health science reports $x 2398-8835 $g Roč. 7, č. 9 (2024), s. e70046
856    41
$u https://pubmed.ncbi.nlm.nih.gov/39323462 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y - $z 0
990    __
$a 20241008 $b ABA008
991    __
$a 20241016082008 $b ABA008
999    __
$a ok $b bmc $g 2196369 $s 1229712
BAS    __
$a 3
BAS    __
$a PreBMC-PubMed-not-MEDLINE
BMC    __
$a 2024 $b 7 $c 9 $d e70046 $e 20240925 $i 2398-8835 $m Health science reports $n Health Sci Rep $x MED00207111
LZP    __
$a Pubmed-20241008

Najít záznam

Citační ukazatele

Možnosti archivace