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MiR-155 deficiency and hypoxia results in metabolism switch in the leukemic B-cells

E. Golovina, T. Heizer, L. Daumova, M. Bajecny, S. Fontana, V. Griggio, R. Jones, M. Coscia, C. Riganti, K. Savvulidi Vargova

. 2024 ; 24 (1) : 251. [pub] 20240718

Status neindexováno Jazyk angličtina Země Anglie, Velká Británie

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc24018210

Grantová podpora
START/MED/089 and PRIMUS/17/MED/16 Elena Golovina
START/MED/089 Tomas Heizer
START/MED/089 Lenka Daumova
PROGRES Q26/LF1 Martin Bajecny
IG21408 Simona Fontana
IG21408 Valentina Griggio
IG21408 Rebecca Jones
IG21408 Marta Coscia
IG21408 Chiara Riganti
START/MED/089 and PRIMUS/17/MED/16 Karina Savvulidi Vargova

Hypoxia represents one of the key factors that stimulates the growth of leukemic cells in their niche. Leukemic cells in hypoxic conditions are forced to reprogram their original transcriptome, miRNome, and metabolome. How the coupling of microRNAs (miRNAs)/mRNAs helps to maintain or progress the leukemic status is still not fully described. MiRNAs regulate practically all biological processes within cells and play a crucial role in the development/progression of leukemia. In the present study, we aimed to uncover the impact of hsa-miR-155-5p (miR-155, MIR155HG) on the metabolism, proliferation, and mRNA/miRNA network of human chronic lymphocytic leukemia cells (CLL) in hypoxic conditions. As a model of CLL, we used the human MEC-1 cell line where we deleted mature miR-155 with CRISPR/Cas9. We determined that miR-155 deficiency in leukemic MEC-1 cells results in lower proliferation even in hypoxic conditions in comparison to MEC-1 control cells. Additionally, in MEC-1 miR-155 deficient cells we observed decreased number of populations of cells in S phase. The miR-155 deficiency under hypoxic conditions was accompanied by an increased apoptosis. We detected a stimulatory effect of miR-155 deficiency and hypoxia at the level of gene expression, seen in significant overexpression of EGLN1, GLUT1, GLUT3 in MEC-1 miR-155 deficient cells. MiR-155 deficiency and hypoxia resulted in increase of glucose and lactate uptake. Pyruvate, ETC and ATP were reduced. To conclude, miR-155 deficiency and hypoxia affects glucose and lactate metabolism by stimulating the expression of glucose transporters as GLUT1, GLUT3, and EGLN1 [Hypoxia-inducible factor prolyl hydroxylase 2 (HIF-PH2)] genes in the MEC-1 cells.

Citace poskytuje Crossref.org

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