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The Antigen-Specific Response of NK Cells to SARS-CoV-2 Correlates With KIR2DS4 Expression
MO. Ustiuzhanina, AA. Boyko, JD. Vavilova, AE. Siniavin, MA. Streltsova, IV. Astrakhantseva, MS. Drutskaya, DM. Chudakov, EI. Kovalenko
Jazyk angličtina Země Spojené státy americké
Typ dokumentu časopisecké články
Grantová podpora
This research was funded by the Russian Science Foundation grant No 22-15-00503. Experiments using MagPix were performed at the Sirius University of Science and Technology and supported by the Ministry of Science and Higher Education of the Russian Federation (Agreement 075-10-2021-093).
PubMed
39540437
DOI
10.1002/jmv.70057
Knihovny.cz E-zdroje
- MeSH
- aktivace lymfocytů MeSH
- buňky NK * imunologie MeSH
- COVID-19 * imunologie MeSH
- degranulace buněk MeSH
- dospělí MeSH
- imunoglobulin G MeSH
- imunologická paměť MeSH
- interferon gama * imunologie metabolismus MeSH
- lidé středního věku MeSH
- lidé MeSH
- protilátky virové krev imunologie MeSH
- receptory KIR genetika metabolismus MeSH
- SARS-CoV-2 * imunologie MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
Natural killer (NK) cells play a pivotal role in the immune response against viral infections, including SARS-CoV-2. However, our understanding of memory NK cell responses in the context of SARS-CoV-2 remains limited. To address this, we investigated the memory-like response of NK cells to SARS-CoV-2 peptides, presented by autologous cells. Blood samples from 45 donors underwent analysis for SARS-CoV-2 IgG antibodies, categorizing them into four groups based on the antibody kind and level. NK cells from SARS-CoV-2-experienced donors demonstrated enhanced degranulation and activation levels, IFNγ production and proliferative potential in response to SARS-CoV-2 peptides. Investigation of highly proliferating NK cells demonstrated the formation of distinct clusters depending on the SARS-CoV-2 peptide supplementation and the donor group. RNA sequencing revealed differential gene expression patterns, highlighting metabolism, protein transport, and immune response genes. Notably, KIR2DS4 expression correlated with enhanced IFNγ production, degranulation and proliferation levels, suggesting a role in SARS-CoV-2 recognition. Collectively, these findings provide detailed insights into antigen-specific NK cell responses to SARS-CoV-2 peptides, indicating potential mechanisms underlying NK cell activation in antiviral immunity.
Central European Institute of Technology Masaryk University Brno Czech Republic
Department Bioinformatics Abu Dhabi Stem Cell Center Al Muntazah Abu Dhabi United Arab Emirates
Citace poskytuje Crossref.org
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