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Immunologically active cell wall polysaccharides of green microalga Dictyosphaerium chlorelloides (Chlorellacea)
L. Sushytskyi, A. Synytsya, P. Lukáč, L. Rajsiglová, P. Capek, R. Pohl, R. Bleha, LE. Vannucci, D. Smrz, J. Čopíková, P. Kaštánek
Jazyk angličtina Země Anglie, Velká Británie
Typ dokumentu časopisecké články
- MeSH
- adjuvancia imunologická farmakologie chemie izolace a purifikace MeSH
- buněčná stěna * chemie MeSH
- Chlorophyta chemie MeSH
- mikrořasy * chemie MeSH
- myši inbrední BALB C MeSH
- myši MeSH
- polysacharidy * farmakologie chemie izolace a purifikace MeSH
- slezina cytologie účinky léků MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
Dictyosphaerium chlorelloides is a green microalga from the Chlorella clade that produces highly viscous exocellular polysaccharides. The cell wall polysaccharides of this alga have not been studied in detail. In this article, water-soluble polysaccharides from D. chlorelloides biomass were extracted with hot water and purified by preparative chromatography. The composition, structural features and molecular masses of subsequently eluted fractions F1, F2, F3, F4 and F5 (minor) were determined. Three high-yield products F1, F3 and F4 consisted mainly of galactopyranosyl, 2-O-methyl-galactopyranosyl, rhamnopyranosyl and mannopyranosyl units at different proportions, while F2 was rich in glucose. Immunoactivity of these fractions was evidenced in a mixed population of immune cells derived from mice spleens after incubation with polysaccharides by flow cytometry, MTT and Immunospot assays. These fractions, except F2, demonstrated selective immunostimulant activity, and the F1 fraction induced the most potent effect, closely followed by the F3 and F4 fractions. The in vivo mechanism of their action is associated with the activation of innate immunity and shapes the immune response to the Th1 type.
EcoFuel Laboratories s r o Ocelářská 9 Prague 9 Libeň 190 00 Czech Republic
Institute of Chemistry Slovak Academy of Sciences Dúbravská cesta 9 845 38 Bratislava Slovakia
Institute of Organic Chemistry and Biochemistry AS CR Flemingovo sq 2 166 28 Prague 6 Czech Republic
Citace poskytuje Crossref.org
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- $a Sushytskyi, Leonid $u Department of Carbohydrates and Cereals, Faculty of Food and Biochemical Technology, University of Chemistry and Technology in Prague, Technická 5, 166 28, Prague 6 Dejvice, Czech Republic; Laboratory of Immunotherapy, Institute of Microbiology, Czech Academy of Sciences, Vídeňská 1083, 142 20, Prague 4 Krč, Czech Republic
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- $a Dictyosphaerium chlorelloides is a green microalga from the Chlorella clade that produces highly viscous exocellular polysaccharides. The cell wall polysaccharides of this alga have not been studied in detail. In this article, water-soluble polysaccharides from D. chlorelloides biomass were extracted with hot water and purified by preparative chromatography. The composition, structural features and molecular masses of subsequently eluted fractions F1, F2, F3, F4 and F5 (minor) were determined. Three high-yield products F1, F3 and F4 consisted mainly of galactopyranosyl, 2-O-methyl-galactopyranosyl, rhamnopyranosyl and mannopyranosyl units at different proportions, while F2 was rich in glucose. Immunoactivity of these fractions was evidenced in a mixed population of immune cells derived from mice spleens after incubation with polysaccharides by flow cytometry, MTT and Immunospot assays. These fractions, except F2, demonstrated selective immunostimulant activity, and the F1 fraction induced the most potent effect, closely followed by the F3 and F4 fractions. The in vivo mechanism of their action is associated with the activation of innate immunity and shapes the immune response to the Th1 type.
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- $a Synytsya, Andriy $u Department of Carbohydrates and Cereals, Faculty of Food and Biochemical Technology, University of Chemistry and Technology in Prague, Technická 5, 166 28, Prague 6 Dejvice, Czech Republic. Electronic address: sinicaa@vscht.cz
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