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Analysis of extracellular vesicles of frequently used colorectal cancer cell lines

M. Boudná, N. Blavet, T. Samoilenko, T. Macháčková, R. Jugas, P. Vychytilová-Faltejsková, M. Boudný, R. Bartošová, J. Kotouček, V. Bystrý, K. Koželková, O. Slabý, K. Součková

. 2025 ; 25 (1) : 555. [pub] 20250327

Language English Country England, Great Britain

Document type Journal Article

Persistent link   https://www.medvik.cz/link/bmc25009424

Grant support
20-18889S Grantová Agentura České Republiky
20-18889S Grantová Agentura České Republiky
20-18889S Grantová Agentura České Republiky
LX22NPO5102 National Institute for Cancer Research (Program EXCELES)-Funded by the European Union-Next Generation EU
LX22NPO5102 National Institute for Cancer Research (Program EXCELES)-Funded by the European Union-Next Generation EU
LX22NPO5102 National Institute for Cancer Research (Program EXCELES)-Funded by the European Union-Next Generation EU
LX22NPO5102 National Institute for Cancer Research (Program EXCELES)-Funded by the European Union-Next Generation EU
LX22NPO5102 National Institute for Cancer Research (Program EXCELES)-Funded by the European Union-Next Generation EU
LX22NPO5102 National Institute for Cancer Research (Program EXCELES)-Funded by the European Union-Next Generation EU

Colorectal cancer (CRC) ranks as the second most prevalent malignancy globally, highlighting the urgent need for more effective diagnostic and therapeutic strategies, as well as a deeper understanding of its molecular basis. Extensive research has demonstrated that cells actively secrete extracellular vesicles (EVs) to mediate intercellular communication at both proximal and distal sites. In this study, we conducted a comprehensive analysis of the RNA content of small extracellular vesicles (sEVs) secreted into the culture media of five frequently utilised CRC cell lines (RKO, HCT116, HCT15, HT29, and DLD1). RNA sequencing data revealed significant insights into the RNA profiles of these sEVs, identifying nine protein-coding genes and fourteen long non-coding RNA (lncRNA) genes that consistently ranked among the top 30 most abundant across all cell lines. Notably, the genes found in sEVs were highly similar among the cell lines, indicating a conserved molecular signature. Several of these genes have been previously documented in the context of cancer biology, while others represent novel discoveries. These findings provide valuable insights into the molecular cargo of sEVs in CRC, potentially unveiling novel biomarkers and therapeutic targets.

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$a Colorectal cancer (CRC) ranks as the second most prevalent malignancy globally, highlighting the urgent need for more effective diagnostic and therapeutic strategies, as well as a deeper understanding of its molecular basis. Extensive research has demonstrated that cells actively secrete extracellular vesicles (EVs) to mediate intercellular communication at both proximal and distal sites. In this study, we conducted a comprehensive analysis of the RNA content of small extracellular vesicles (sEVs) secreted into the culture media of five frequently utilised CRC cell lines (RKO, HCT116, HCT15, HT29, and DLD1). RNA sequencing data revealed significant insights into the RNA profiles of these sEVs, identifying nine protein-coding genes and fourteen long non-coding RNA (lncRNA) genes that consistently ranked among the top 30 most abundant across all cell lines. Notably, the genes found in sEVs were highly similar among the cell lines, indicating a conserved molecular signature. Several of these genes have been previously documented in the context of cancer biology, while others represent novel discoveries. These findings provide valuable insights into the molecular cargo of sEVs in CRC, potentially unveiling novel biomarkers and therapeutic targets.
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