-
Je něco špatně v tomto záznamu ?
Disruption of Extracellular Matrix and Perineuronal Nets Modulates Extracellular Space Volume and Geometry
E. Syková, I. Voříšek, Z. Starčuk, J. Kratochvíla, I. Pavlova, Y. Ichikawa, JCF. Kwok, E. Kmoníčková, S. Myronchenko, T. Hromádka, T. Smolek, M. Avila, N. Basheer, N. Žilka
Jazyk angličtina Země Spojené státy americké
Typ dokumentu časopisecké články
NLK
Open Access Digital Library
od 1981-01-01
Open Access Digital Library
od 1981-01-01
- MeSH
- astrocyty metabolismus MeSH
- chondroitinsulfát proteoglykany metabolismus MeSH
- extracelulární matrix * metabolismus MeSH
- extracelulární prostor * metabolismus MeSH
- gliový fibrilární kyselý protein metabolismus MeSH
- hymekromon farmakologie MeSH
- krysa rodu rattus MeSH
- kyselina hyaluronová MeSH
- mozek metabolismus MeSH
- nervová síť účinky léků diagnostické zobrazování MeSH
- potkani Sprague-Dawley MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
Extracellular matrix (ECM) is a network of macromolecules which has two forms-perineuronal nets (PNNs) and a diffuse ECM (dECM)-both influence brain development, synapse formation, neuroplasticity, CNS injury and progression of neurodegenerative diseases. ECM remodeling can influence extrasynaptic transmission, mediated by diffusion of neuroactive substances in the extracellular space (ECS). In this study we analyzed how disrupted PNNs and dECM influence brain diffusibility. Two months after oral treatment of rats with 4-methylumbelliferone (4-MU), an inhibitor of hyaluronan (HA) synthesis, we found downregulated staining for PNNs, HA, chondroitin sulfate proteoglycans, and glial fibrillary acidic protein. These changes were enhanced after 4 and 6 months and were reversible after a normal diet. Morphometric analysis further indicated atrophy of astrocytes. Using real-time iontophoretic method dysregulation of ECM resulted in increased ECS volume fraction α in the somatosensory cortex by 35%, from α = 0.20 in control rats to α = 0.27 after the 4-MU diet. Diffusion-weighted magnetic resonance imaging revealed a decrease of mean diffusivity and fractional anisotropy (FA) in the cortex, hippocampus, thalamus, pallidum, and spinal cord. This study shows the increase in ECS volume, a loss of FA, and changes in astrocytes due to modulation of PNNs and dECM that could affect extrasynaptic transmission, cell-to-cell communication, and neural plasticity.
Department of Pharmacology 2nd Faculty of Medicine Charles University Prague 15000 Czech Republic
Institute of Neuroimmunology Slovak Academy of Science Bratislava 84510 Slovakia
Institute of Scientific Instruments Czech Academy of Sciences Brno 61200 Czech Republic
Citace poskytuje Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc25009853
- 003
- CZ-PrNML
- 005
- 20250429134715.0
- 007
- ta
- 008
- 250415s2025 xxu f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1523/JNEUROSCI.0517-24.2024 $2 doi
- 035 __
- $a (PubMed)39753300
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a xxu
- 100 1_
- $a Syková, Eva $u Institute of Neuroimmunology, Slovak Academy of Science, Bratislava 84510, Slovakia sykovae@gmail.com $1 https://orcid.org/0000000301790182 $7 jn20000710633
- 245 10
- $a Disruption of Extracellular Matrix and Perineuronal Nets Modulates Extracellular Space Volume and Geometry / $c E. Syková, I. Voříšek, Z. Starčuk, J. Kratochvíla, I. Pavlova, Y. Ichikawa, JCF. Kwok, E. Kmoníčková, S. Myronchenko, T. Hromádka, T. Smolek, M. Avila, N. Basheer, N. Žilka
- 520 9_
- $a Extracellular matrix (ECM) is a network of macromolecules which has two forms-perineuronal nets (PNNs) and a diffuse ECM (dECM)-both influence brain development, synapse formation, neuroplasticity, CNS injury and progression of neurodegenerative diseases. ECM remodeling can influence extrasynaptic transmission, mediated by diffusion of neuroactive substances in the extracellular space (ECS). In this study we analyzed how disrupted PNNs and dECM influence brain diffusibility. Two months after oral treatment of rats with 4-methylumbelliferone (4-MU), an inhibitor of hyaluronan (HA) synthesis, we found downregulated staining for PNNs, HA, chondroitin sulfate proteoglycans, and glial fibrillary acidic protein. These changes were enhanced after 4 and 6 months and were reversible after a normal diet. Morphometric analysis further indicated atrophy of astrocytes. Using real-time iontophoretic method dysregulation of ECM resulted in increased ECS volume fraction α in the somatosensory cortex by 35%, from α = 0.20 in control rats to α = 0.27 after the 4-MU diet. Diffusion-weighted magnetic resonance imaging revealed a decrease of mean diffusivity and fractional anisotropy (FA) in the cortex, hippocampus, thalamus, pallidum, and spinal cord. This study shows the increase in ECS volume, a loss of FA, and changes in astrocytes due to modulation of PNNs and dECM that could affect extrasynaptic transmission, cell-to-cell communication, and neural plasticity.
- 650 _2
- $a zvířata $7 D000818
- 650 12
- $a extracelulární matrix $x metabolismus $7 D005109
- 650 _2
- $a krysa rodu Rattus $7 D051381
- 650 _2
- $a mužské pohlaví $7 D008297
- 650 12
- $a extracelulární prostor $x metabolismus $7 D005110
- 650 _2
- $a hymekromon $x farmakologie $7 D006923
- 650 _2
- $a nervová síť $x účinky léků $x diagnostické zobrazování $7 D009415
- 650 _2
- $a potkani Sprague-Dawley $7 D017207
- 650 _2
- $a kyselina hyaluronová $7 D006820
- 650 _2
- $a astrocyty $x metabolismus $7 D001253
- 650 _2
- $a mozek $x metabolismus $7 D001921
- 650 _2
- $a chondroitinsulfát proteoglykany $x metabolismus $7 D011508
- 650 _2
- $a gliový fibrilární kyselý protein $x metabolismus $7 D005904
- 655 _2
- $a časopisecké články $7 D016428
- 700 1_
- $a Voříšek, Ivan $u Institute of Neuroimmunology, Slovak Academy of Science, Bratislava 84510, Slovakia
- 700 1_
- $a Starčuk, Zenon $u Institute of Scientific Instruments, Czech Academy of Sciences, Brno 61200, Czech Republic
- 700 1_
- $a Kratochvíla, Jiří $u Institute of Scientific Instruments, Czech Academy of Sciences, Brno 61200, Czech Republic
- 700 1_
- $a Pavlova, Iveta $u Institute of Scientific Instruments, Czech Academy of Sciences, Brno 61200, Czech Republic
- 700 1_
- $a Ichikawa, Yuki $u School of Biomedical Sciences, Faculty of Biological Sciences, University of Leeds, Leeds LS2 9JT, United Kingdom
- 700 1_
- $a Kwok, Jessica C F $u School of Biomedical Sciences, Faculty of Biological Sciences, University of Leeds, Leeds LS2 9JT, United Kingdom
- 700 1_
- $a Kmoníčková, Eva $u Department of Pharmacology, Second Faculty of Medicine, Charles University, Prague 15000, Czech Republic
- 700 1_
- $a Myronchenko, Svitlana $u Department of Pharmacology, Second Faculty of Medicine, Charles University, Prague 15000, Czech Republic $1 https://orcid.org/0000000253672834
- 700 1_
- $a Hromádka, Tomáš $u Institute of Neuroimmunology, Slovak Academy of Science, Bratislava 84510, Slovakia
- 700 1_
- $a Smolek, Tomáš $u Institute of Neuroimmunology, Slovak Academy of Science, Bratislava 84510, Slovakia
- 700 1_
- $a Avila, Martin $u Institute of Histology and Embryology of Mendoza, National University of Cuyo, National Scientific and Technical Research Council, Mendoza 5500, Argentina
- 700 1_
- $a Basheer, Neha $u Institute of Neuroimmunology, Slovak Academy of Science, Bratislava 84510, Slovakia $1 https://orcid.org/0000000336693825
- 700 1_
- $a Žilka, Norbert $u Institute of Neuroimmunology, Slovak Academy of Science, Bratislava 84510, Slovakia
- 773 0_
- $w MED00002840 $t The Journal of neuroscience $x 1529-2401 $g Roč. 45, č. 8 (2025)
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/39753300 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y - $z 0
- 990 __
- $a 20250415 $b ABA008
- 991 __
- $a 20250429134711 $b ABA008
- 999 __
- $a ok $b bmc $g 2311318 $s 1246934
- BAS __
- $a 3
- BAS __
- $a PreBMC-MEDLINE
- BMC __
- $a 2025 $b 45 $c 8 $e 20250219 $i 1529-2401 $m The Journal of neuroscience $n J Neurosci $x MED00002840
- LZP __
- $a Pubmed-20250415
