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Proteorhodopsin insights into the molecular mechanism of vectorial proton transport
S. Bukhdruker, I. Gushchin, V. Shevchenko, K. Kovalev, V. Polovinkin, F. Tsybrov, R. Astashkin, A. Alekseev, A. Mikhaylov, S. Bukhalovich, D. Bratanov, Y. Ryzhykau, D. Kuklina, N. Caramello, T. Rokitskaya, Y. Antonenko, M. Rulev, C. Stoev, D....
Language English Country United States
Document type Journal Article
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PubMed Central
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Open Access Digital Library
from 2015-01-01
- MeSH
- Protein Conformation MeSH
- Models, Molecular MeSH
- Proton Pumps chemistry metabolism MeSH
- Protons * MeSH
- Rhodopsins, Microbial * chemistry metabolism MeSH
- Hydrogen Bonding MeSH
- Publication type
- Journal Article MeSH
Bacterial proton pumps, proteorhodopsins (PRs), are a major group of light-driven membrane proteins found in marine bacteria. They are functionally and structurally distinct from archaeal and eukaryotic proton pumps. To elucidate the proton transfer mechanism by PRs and understand the differences to nonbacterial pumps on a molecular level, high-resolution structures of PRs' functional states are needed. In this work, we have determined atomic-resolution structures of MAR, a PR from marine actinobacteria, in various functional states, notably the challenging late O intermediate state. These data and information from recent atomic-resolution structures on an archaeal outward proton pump bacteriorhodopsin and bacterial inward proton pump xenorhodopsin allow for deducing key universal elements for light-driven proton pumping. First, long hydrogen-bonded chains characterize proton pathways. Second, short hydrogen bonds allow proton storage and inhibit their backflow. Last, the retinal Schiff base is the active proton donor and acceptor to and from hydrogen-bonded chains.
Department of Cell and Molecular Biology Biomedical Centre Uppsala University 75124 Uppsala Sweden
ELI Beamlines Centre ELI ERIC 252 41 Dolní Břežany Czechia
European 10 ray Free Electron Laser GmbH 22869 Schenefeld Germany
Frank Laboratory of Neutron Physics Joint Institute for Nuclear Research 141980 Dubna Russia
Hamburg Outstation c o DESY European Molecular Biology Laboratory 22607 Hamburg Germany
Institut de Biologie Structurale J P Ebel Université Grenoble Alpes CEA CNRS 38000 Grenoble France
Institute for Biophysical Chemistry Medizinische Hochschule Hannover D 30625 Hannover Germany
Institute for Nanostructure and Solid State Physics HARBOR Universität Hamburg 22761 Hamburg Germany
Université Paris Saclay CNRS and Ecole Normale Supérieure Paris Saclay 91190 Gif sur Yvette France
References provided by Crossref.org
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