A method of preparation and application of nitrous acid as a mutagen in Claviceps purpurea
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články
PubMed
992505
DOI
10.1007/bf02876936
Knihovny.cz E-zdroje
- MeSH
- Claviceps účinky léků MeSH
- dusitany farmakologie MeSH
- kyselina dusitá chemická syntéza farmakologie MeSH
- metody MeSH
- mutageny * MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- dusitany MeSH
- kyselina dusitá MeSH
- mutageny * MeSH
A simple and speedy procedure is described for the preparation and application of nitrous acid in mutagenic experiments. The conventional incubation mixture of sodium nitrite solution with acetate buffer is replaced by an aqueous solution of nitrous acid prepared by running a solution of sodium nitrite through a column of ion exchange. The procedure eliminates also the interference of other substances. Experimental appraisal of the technique was done during the mutagenic improval of the fungus Claviceps purpurea.
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