The isolation of protoplasts of the fission yeast Schizosaccharomyces by Trichoderma viride and snail enzymes
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články
PubMed
1100491
DOI
10.1007/bf02878108
Knihovny.cz E-zdroje
- MeSH
- Ascomycota izolace a purifikace MeSH
- bakteriologické techniky MeSH
- buněčná stěna metabolismus fyziologie MeSH
- hlemýždi enzymologie MeSH
- mitosporické houby enzymologie MeSH
- protoplasty * ultrastruktura MeSH
- regenerace MeSH
- Schizosaccharomyces izolace a purifikace ultrastruktura MeSH
- Trichoderma enzymologie MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
The formation of protoplasts of the fission yeasts Schizosaccharomyces pombe and Schizosaccharomyces versatilis after the combined application of snail enzymes and Trichoderma viride enzymes in an osmotic stabilizer (0.4M KCl, pH 5.5) was studied by light and electron microscopy. The effect of the enzymes used leads during 30 min to the formation of 100% protoplast population. Using electron microscopy no original walls or wall remnants were detected in the suspension of protoplasts. Protoplasts are viable and in liquid nutrient medium they regenerate cell walls and revert into normal cells. Such a protoplast population may be useful for biochemical study of protoplast metabolism by quantitative methods as well as for the chemical study of regenerating cell walls.
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