Expression of chimeric proteins encoded by the fused BLV reverse transcriptase: beta-galactosidase in Escherichia coli
Jazyk angličtina Země Česko Médium print
Typ dokumentu časopisecké články
PubMed
1282472
Knihovny.cz E-zdroje
- MeSH
- beta-galaktosidasa genetika MeSH
- Escherichia coli MeSH
- exprese genu genetika MeSH
- genetické vektory genetika MeSH
- geny pol genetika MeSH
- plazmidy MeSH
- rekombinantní fúzní proteiny biosyntéza genetika MeSH
- reverzní transkriptasa genetika MeSH
- virus bovinní leukemie genetika MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- beta-galaktosidasa MeSH
- rekombinantní fúzní proteiny MeSH
- reverzní transkriptasa MeSH
The gene for bovine leukemia virus (BLV) reverse transcriptase was cloned in prokaryotic expression vector pUC8-2. After fusion of Escherichia coli lacZ gene to different parts of reverse transcriptase we detected expression of new proteins with molecular weights corresponding to the size of the hybrid genes. A coding region most probably responsible for about a hundred-fold decrease in expression of long fusion proteins has been identified. A few possible causes of this phenomenon were tested.
