A method for the preparation of purified antigens of coxsackievirus B3 from a large volume of cell culture supernatant
Language English Country Switzerland Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
1364026
Knihovny.cz E-resources
- MeSH
- Antigens, Viral isolation & purification MeSH
- Centrifugation, Density Gradient MeSH
- Enterovirus B, Human immunology isolation & purification MeSH
- Hemagglutination Tests MeSH
- Culture Media MeSH
- Polyethylene Glycols MeSH
- Vero Cells microbiology MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Antigens, Viral MeSH
- Culture Media MeSH
- Polyethylene Glycols MeSH
A simple procedure was used for the concentration and partial purification of coxsackievirus B3 (Nancy strain). For a large-scale production of virus. Vero cells grown in roller bottles were used. Virus concentrate from a large volume of cell culture supernatant was prepared by precipitation with 6% (w/w) polyethylene glycol. This crude antigen was further purified by banding in cesium chloride gradient using ultracentrifugation. The infectivity and haemagglutination activity of virus were checked up during the whole procedure and the final recovery of infections virus was about 60%.
