A simple procedure was used for the concentration and partial purification of coxsackievirus B3 (Nancy strain). For a large-scale production of virus. Vero cells grown in roller bottles were used. Virus concentrate from a large volume of cell culture supernatant was prepared by precipitation with 6% (w/w) polyethylene glycol. This crude antigen was further purified by banding in cesium chloride gradient using ultracentrifugation. The infectivity and haemagglutination activity of virus were checked up during the whole procedure and the final recovery of infections virus was about 60%.

Institute of Sera and Vaccines Prague Czechoslovakia