Quality control program of steroid receptor assays: an international study
Language English Country Slovakia Media print
Document type Comparative Study, Journal Article
PubMed
1382235
Knihovny.cz E-resources
- MeSH
- Chemistry Techniques, Analytical standards MeSH
- Cytosol chemistry MeSH
- Dextrans MeSH
- Charcoal MeSH
- Immunohistochemistry MeSH
- International Cooperation MeSH
- Receptors, Estrogen analysis MeSH
- Receptors, Progesterone analysis MeSH
- Reproducibility of Results MeSH
- Quality Control MeSH
- Cattle MeSH
- Receptors, Steroid analysis MeSH
- Uterus ultrastructure MeSH
- Animals MeSH
- Check Tag
- Cattle MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Comparative Study MeSH
- Names of Substances
- Dextrans MeSH
- Charcoal MeSH
- Receptors, Estrogen MeSH
- Receptors, Progesterone MeSH
- Receptors, Steroid MeSH
Lyophilized calf uterine cytosol standards were prepared for quality control of estrogen receptor (ER) determination, and lyophilized cytosols and tissue powders were used for quality control of progesterone receptor (PR) analysis. Two series of four samples were analyzed either for ER or PR contents, twice within one month, by 7 laboratories in 5 countries. Coefficient of variation (CV) of the between-laboratory averages assayed in a single run of ER-positive (ER+) and PR-positive (PR+) standards varied from 29.6 to 61.8% and from 32.4 to 76.2%, respectively. All laboratories, with the exception of a single value, could recognize samples of low, medium, an high ER level, as well as a negative sample. Most laboratories evaluated properly also the level of PR samples. The average between-laboratory CV values of protein determination in the relevant standards were 23%.
