Easy paraffine technique in processing of bone marrow samples is convenient for structural evaluation and for immunohistology as well. A short fixation in 10% formalin will do, especially when combined with decalcination in Chelaton III. Preparation of sections, staining and immunohistology are analogous to other tissues. Nevertheless, it would be better try to dilute antibodies higher than is usually recommended even if a longer incubation were needed. Intensity of staining can be increased by performing lead sulphocyanate treatment in a microwave oven.

Hlavův 1 patologicko anatomický ústav 1 LF UK Praha