Fragments of Candida boidinii chromosomal DNA were inserted into the integrative vector YIp-kanr and examined for the presence of sequences promoting autonomous replication of plasmids in Saccharomyces cerevisiae. Restriction maps of two plasmids, designated S6/4 and S6/5, originating from the same S. cerevisiae transformant, were constructed. Southern hybridization data confirmed that the plasmids carry sequences from the C. boidinii chromosome. Both plasmids transform S. cerevisiae strains at 4-5-fold higher frequency than cloning vectors based on the replication origin of the 2 microns plasmid. Mitotic stability of the constructed plasmids is similar to that of the 2 mu-based vector pNF2 in S. cerevisiae.

Institute of Microbiology Czechoslovak Academy of Sciences Prague

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J Mol Biol. 1977 Jun 15;113(1):237-51 PubMed

Nucleic Acids Res. 1981 Jul 10;9(13):2989-98 PubMed

Proc Natl Acad Sci U S A. 1979 Mar;76(3):1035-9 PubMed

Gene. 1983 Oct;24(2-3):157-62 PubMed

Gene. 1987;57(2-3):267-72 PubMed

Nucleic Acids Res. 1986 Sep 25;14(18):7504 PubMed

Nature. 1980 Oct 30;287(5785):869-71 PubMed

Mol Cell Biol. 1984 Apr;4(4):651-6 PubMed

Gene. 1979 May;6(1):23-8 PubMed

Folia Microbiol (Praha). 1974;19(4):249-56 PubMed

Methods Cell Biol. 1975;12:39-44 PubMed

J Mol Biol. 1975 Nov 5;98(3):503-17 PubMed

Mol Cell Biol. 1985 Dec;5(12):3376-85 PubMed

J Bacteriol. 1983 Jun;154(3):1033-9 PubMed

Curr Genet. 1986;10(10):741-7 PubMed

Mol Cell Biol. 1986 Jan;6(1):142-9 PubMed

Curr Genet. 1990 Nov;18(4):303-13 PubMed

Gene. 1983 May-Jun;22(2-3):203-9 PubMed

Cold Spring Harb Symp Quant Biol. 1979;43 Pt 2:1305-13 PubMed

Proc Natl Acad Sci U S A. 1980 Aug;77(8):4559-63 PubMed

Gene. 1986;44(1):171-5 PubMed