Transformation of Streptomyces lincolnensis protoplasts with plasmid vectors
Language English Country United States Media print
Document type Journal Article
PubMed
1505879
DOI
10.1007/bf02933144
Knihovny.cz E-resources
- MeSH
- Transformation, Bacterial * MeSH
- Genetic Vectors MeSH
- Nucleic Acid Hybridization MeSH
- Plasmids * MeSH
- Protoplasts metabolism MeSH
- Blotting, Southern MeSH
- Streptomyces genetics growth & development MeSH
- Publication type
- Journal Article MeSH
A method for the preparation and regeneration of protoplasts of Streptomyces lincolnensis is described. Mycelium in the early exponential phase appeared to be most suitable for this purpose and yielded up to 25% regenerated intact cells. Transformation of S. lincolnensis protoplasts was achieved using broad-host-range streptomycete plasmid vectors pIJ622, pMP66, pRS410 and pIJ943 constructed from replicons pIJ101, pSLG33 and SCP2. The efficiency of transformation was 3.10(3) transformants per micrograms plasmid DNA when (2-5).10(7) recipient protoplasts were used. Interspecific transformations showed that there is no efficient restriction system in S. lincolnensis that would limit the transfer of genetic information from S. lividans or E. coli.
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