Transformation of Streptomyces granaticolor with natural and recombinant plasmid vectors
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články
PubMed
3002927
DOI
10.1007/bf02927609
Knihovny.cz E-zdroje
- MeSH
- bakteriální transformace * MeSH
- plazmidy * MeSH
- rekombinace genetická * MeSH
- restrikční enzymy MeSH
- spory bakteriální MeSH
- Streptomyces genetika růst a vývoj fyziologie MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- restrikční enzymy MeSH
Protoplasts of Streptomyces granaticolor were found to be transformable by the broad-host-range plasmid pIJ350 but no transformants were detected when the narrow-host-range plasmid pIJ2 or the shuttle vector pPM66 (pIJ350--pBR322) isolated from E. coli cells were used. The onset of blue colour granaticin production by S. granaticolor cells was used as a marker to prepare protoplasts with a high transformation capacity. The presence of a restriction system is discussed.
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