Field utility of phenolic glycolipid coated latex agglutination test for rapid detection of bacilliferous leprosy cases
Language English Country Czech Republic Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
1512452
Knihovny.cz E-resources
- MeSH
- Agglutination Tests methods MeSH
- Antigens, Bacterial * MeSH
- Glycolipids * MeSH
- Latex MeSH
- Leprosy blood diagnosis MeSH
- Humans MeSH
- Microspheres MeSH
- Mycobacterium leprae immunology MeSH
- Antibodies, Bacterial immunology MeSH
- Sensitivity and Specificity MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Antigens, Bacterial * MeSH
- Glycolipids * MeSH
- Latex MeSH
- phenolic glycolipid I, Mycobacterium leprae MeSH Browser
- Antibodies, Bacterial MeSH
Serum samples were collected from eighty-three leprosy patients and twenty-five healthy controls supposedly not exposed to Mycobacterium leprae infection. Phenolic glycolipid-1 coated latex agglutination test (PGL-LAT) was carried out with the serum samples to detect antibodies specific to M. leprae. Samples showing positive agglutination were 50% in the lepromatous leprosy (LL) group showing no erythema nodosum leprosum (ENL) complications, 66.6% in LL group with ENL complication, 60% in borderline lepromatous (BL) group, 50% in borderline (BB) and 33.3% in borderline tuberculoid (BT). The patients belonging to the tuberculoid (TT) group and most of the long-term treated patients were interestingly negative, and so were sera from all the healthy controls. PGL-LAT developed by us therefore is specific and a fairly sensitive technique to detect antibodies specific to M. leprae and will be very useful in field conditions.
