Black substrate for spectrophotometric determination of cellulase activity in coloured solutions
Jazyk angličtina Země Nizozemsko Médium print
Typ dokumentu časopisecké články
PubMed
1770200
DOI
10.1016/0165-022x(91)90005-h
PII: 0165-022X(91)90005-H
Knihovny.cz E-zdroje
- MeSH
- celulasa analýza MeSH
- epichlorhydrin MeSH
- filtrace MeSH
- hydrolýza MeSH
- inkoust * MeSH
- kinetika MeSH
- reagencia zkříženě vázaná MeSH
- rozpustnost MeSH
- roztoky MeSH
- sodná sůl karboxymethylcelulosy chemie MeSH
- spektrofotometrie * metody MeSH
- substrátová specifita MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- celulasa MeSH
- epichlorhydrin MeSH
- reagencia zkříženě vázaná MeSH
- roztoky MeSH
- sodná sůl karboxymethylcelulosy MeSH
A simple procedure for spectrophotometric determination of cellulase activity in coloured solutions is described. CM-cellulose, cross-linked with epichlorhydrin in the presence of black drawing ink, is used as an insoluble chromolytic substrate. The absorbance of reaction mixture filtrates are read in the near infra-red region (at 800-900 nm) where the absorbances of contaminating coloured substances are substantially lowered; by contrast, black drawing ink released from the substrate during the action of cellulases absorbs strongly at these wavelengths.
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