Localization and characterization of a temporally regulated promoter from the Streptomyces aureofaciens 2201 plasmid pSA 2201
Jazyk angličtina Země Nizozemsko Médium print
Typ dokumentu časopisecké články
PubMed
1840494
DOI
10.1016/0167-4781(91)90160-n
PII: 0167-4781(91)90160-N
Knihovny.cz E-zdroje
- MeSH
- autoradiografie MeSH
- bakteriální geny MeSH
- DNA řízené RNA-polymerasy genetika MeSH
- elektroforéza v polyakrylamidovém gelu MeSH
- genetická transkripce MeSH
- hybridizace nukleových kyselin MeSH
- molekulární sekvence - údaje MeSH
- northern blotting MeSH
- plazmidy MeSH
- promotorové oblasti (genetika) * MeSH
- regulace genové exprese u bakterií MeSH
- sekvence aminokyselin MeSH
- sekvenční homologie nukleových kyselin MeSH
- Streptomyces aureofaciens enzymologie genetika MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- DNA řízené RNA-polymerasy MeSH
An RNA polymerase-binding 167 bp HinfI fragment from a low-copy Streptomyces plasmid pSA 2201 has been shown to have promoter activity in vivo using a promoter-probe vector. This promoter (A1) is probably involved in expression of the genes responsible for the production of an antibiotic compound, found to be located on this plasmid. A 2600 nucleotides (nt) long transcript starting from this promoter has been identified by Northern hybridization analysis. The transcription start point has been determined using high-resolution S1 mapping and confirmed by in vitro transcription analysis with purified S. aureofaciens 2201 RNA polymerase. The A1 promoter shows no homology in the -10 and -35 consensus sequence of the typical bacterial promoters, and expression from this promoter is temporally dependent on the phase of growth having the maximum transcription activity in the stationary phase.
Citace poskytuje Crossref.org
GENBANK
M75971, M75972, M75973, M75974, M75975, S57575, S57577, S57579, S59357, X58025
