Characterization of an asporogenous mutant of Streptomyces aureofaciens with increased resistance to several aminoglycoside antibiotics
Language English Country United States Media print
Document type Journal Article
PubMed
1841849
DOI
10.1007/bf02935822
Knihovny.cz E-resources
- MeSH
- Aminoglycosides MeSH
- Anti-Bacterial Agents pharmacology MeSH
- Drug Resistance, Microbial genetics MeSH
- Bacterial Proteins genetics isolation & purification MeSH
- Mutation MeSH
- Spores, Bacterial genetics MeSH
- Streptomyces aureofaciens drug effects genetics ultrastructure MeSH
- Tetracycline biosynthesis MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Aminoglycosides MeSH
- Anti-Bacterial Agents MeSH
- Bacterial Proteins MeSH
- Tetracycline MeSH
An asporogenous spontaneous mutant of Streptomyces aureofaciens named ASR1 was selected on streptomycin gradient plates. The mutant is very stable and differs in ultrastructure and morphology, it is prototrophic but it lost the ability to grow well on soybean extract medium and produces one-tenth tetracyclines of the parent. The ASR1 mutant has a 3-4-fold increased resistance to streptomycin and is cross-resistant to other aminoglycosides. Comparison of the protein profiles from both strains on SDS gels revealed a very low expression of a 29.5 kDa protein in the ASR1 mutant which is overexpressed in both vegetative cells and spores of the parental strain.
See more in PubMed
Nature. 1970 Aug 15;227(5259):680-5 PubMed
Biochem J. 1988 Jul 1;253(1):263-7 PubMed
Folia Microbiol (Praha). 1978;23(3):177-82 PubMed
Z Allg Mikrobiol. 1981;21(9):633-42 PubMed
J Basic Microbiol. 1987;27(9):521-8 PubMed
FEMS Microbiol Lett. 1989 Nov;53(1-2):11-5 PubMed
J Biophys Biochem Cytol. 1958 Nov 25;4(6):671-8 PubMed
J Bacteriol. 1984 Mar;157(3):863-7 PubMed
Ann N Y Acad Sci. 1954 Oct 29;60(1):136-51 PubMed
Folia Microbiol (Praha). 1969;14(3):215-25 PubMed
J Am Pharm Assoc Am Pharm Assoc. 1949 Aug;38(8):473-5 PubMed
Z Allg Mikrobiol. 1979;19(6):415-30 PubMed
J Gen Microbiol. 1982 Nov;128(11):2687-91 PubMed
