Distribution of cells in different stages of the cell cycle and some age-related physiological characteristics in dependence on the dilution rate in chemostat cultures of Candida utilis
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články
PubMed
2210493
DOI
10.1007/bf02820492
Knihovny.cz E-zdroje
- MeSH
- buněčný cyklus * MeSH
- Candida analýza cytologie růst a vývoj MeSH
- centrifugace MeSH
- fungální proteiny analýza MeSH
- fungální RNA analýza MeSH
- interfáze MeSH
- mitóza MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- fungální proteiny MeSH
- fungální RNA MeSH
Position of cells in their cell cycle was determined microscopically in chemostat cultures of Candida utilis. Proportion of cells in phase G1 decreased in a linear manner from 86% to 58% with dilution rate. Proportion of cells in phase S increased in the same range of D from 5.6 to 13.5% and in the (G2 + M) phase from 8.4 to 28.5%, again linearly. Differential centrifugation was used to separate chemostat cultures to mother and daughter cells. Analyses showed that, relative to mother cells, daughter cells contain 2.1-11.9% more protein and 25.5-34.6% more RNA in dry matter. Their mass is 34.4-5.6% lower and volume is 154-19% smaller.
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