Neutralization of inhibitory activity derived from cells of MDS patients and ML-2 cell line by monoclonal antibodies against H and L-subunit-rich ferritins
Language English Country Germany Media print
Document type Journal Article
PubMed
2271775
DOI
10.1007/bf01732877
Knihovny.cz E-resources
- MeSH
- Immune Sera immunology MeSH
- Granulocyte-Macrophage Colony-Stimulating Factor pharmacology MeSH
- Macrophage Colony-Stimulating Factor pharmacology MeSH
- Ferritins immunology MeSH
- Culture Media MeSH
- Humans MeSH
- Antibodies, Monoclonal immunology MeSH
- Myelodysplastic Syndromes metabolism pathology MeSH
- Leukemia, Myeloid metabolism pathology MeSH
- Tumor Cells, Cultured MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Immune Sera MeSH
- Granulocyte-Macrophage Colony-Stimulating Factor MeSH
- Macrophage Colony-Stimulating Factor MeSH
- Ferritins MeSH
- Culture Media MeSH
- Antibodies, Monoclonal MeSH
Monoclonal antibody 2A4 against human heart ferritin and a polyclonal antibody against human placental ferritin inactivated the suppressor activity present in media conditioned by cells of patients with myelodysplastic syndrome (MDS). These antibodies also neutralized the inhibitory activity released by cells of myeloid leukemic ML-2 cell line. In contrast, a monoclonal antibody against the ferritin L subunit did not neutralize the inhibitory activity of the media conditioned either by the cells of patients with MDS or by the ML-2 cell line. Neutralization of the inhibitory activity present in supernatants of cells of MDS patients and the ML-2 cell line by monoclonal antibodies against the H subunit of ferritin strongly indicates that the inhibitory activity resides in the acidic isoferritin molecule.
See more in PubMed
Leuk Res. 1980;4(5):437-47 PubMed
Blut. 1987 Sep;55(3):165-71 PubMed
Biochim Biophys Acta. 1986 Jul 25;872(1-2):61-71 PubMed
Blood. 1986 Dec;68(6):1257-63 PubMed
Blood. 1982 Sep;60(3):595-607 PubMed
Blut. 1986 Jan;52(1):51-8 PubMed
Blood. 1986 Mar;67(3):789-95 PubMed
Blood. 1986 Feb;67(2):436-43 PubMed
J Exp Med. 1981 Jun 1;153(6):1426-44 PubMed
J Natl Cancer Inst. 1978 Mar;60(3):497-511 PubMed
Clin Chim Acta. 1983 Nov 15;134(3):347-56 PubMed
Neoplasma. 1989;36(1):83-9 PubMed
