Phosphonate analogues of dinucleotides as substrates for DNA-dependent RNA polymerase from Escherichia coli in primed abortive initiation reaction
Jazyk angličtina Země Nizozemsko Médium print
Typ dokumentu časopisecké články
PubMed
2489057
DOI
10.1016/0141-8130(89)90037-8
PII: 0141-8130(89)90037-8
Knihovny.cz E-zdroje
- MeSH
- dinukleosidfosfáty metabolismus MeSH
- DNA řízené RNA-polymerasy metabolismus MeSH
- elektroforéza v polyakrylamidovém gelu MeSH
- Escherichia coli enzymologie MeSH
- genetická transkripce MeSH
- kinetika MeSH
- organofosfonáty MeSH
- poly dA-dT metabolismus MeSH
- substrátová specifita MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- dinukleosidfosfáty MeSH
- DNA řízené RNA-polymerasy MeSH
- organofosfonáty MeSH
- poly dA-dT MeSH
Dinucleotides (3'-5')-ApU and UpA and their 3'-O-phosphonylmethyl and 5'-O-phosphonylmethyl analogues were studied as substrates in the primed abortive synthesis catalysed by Escherichia coli DNA-dependent RNA polymerase on poly[d(A-T)] template. All phosphonate analogues of dinucleotides containing the anomalous sugar-phosphate backbone are substrates for the holoenzyme as verified by RNase A and RNase T2 digestion of the trinucleotide analogues obtained. The finding that phosphonate dinucleotides act as primers for transcription indicates that steric requirements at the initiation site are not as specific as previously supposed. Analysis of kinetic constants of ordered bibi reaction Kia, KmA, KmB and Vmax suggests that the instability of short RNA-DNA hybrids contributes to the abortive release of trinucleotides formed.
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