To study the possible involvement of mouse mammary tumour virus (MMTV) related agent in human cancer we analysed 300 samples of human sera for the presence of antibodies to MMTV structural proteins. All sera were tested by immunoblotting to achieve high specificity. Out of 300 sera, 22 reacted with transframe protein p30, 16 with the ribonucleoprotein p14, six with the envelope glycoprotein gp52 and three with the major core protein p27. Reactivities to p30 and p14 were observed in sera from cancer patients and healthy controls; reactivities to p27 and gp52 predominated in sera of cancer patients. Sera frequently reacted with a 42 kDa protein which is a cellular contaminant of the virus.

Cancer Research Institute Slovak Academy of Sciences Bratislava Czechoslovakia

See more in PubMed

Int J Cancer. 1980 Jun 15;25(6):721-5 PubMed

Biochem J. 1963 Oct;89:114-23 PubMed

Proc Natl Acad Sci U S A. 1980 Mar;77(3):1617-21 PubMed

Proc Natl Acad Sci U S A. 1980 Mar;77(3):1622-6 PubMed

Anal Biochem. 1980 Mar 1;102(2):459-71 PubMed

Proc Natl Acad Sci U S A. 1981 Apr;78(4):2483-7 PubMed

Cancer. 1981 Jun 1;47(11):2696-703 PubMed

Proc Natl Acad Sci U S A. 1982 Sep;79(18):5503-7 PubMed

Cancer Res. 1983 Jan;43(1):239-44 PubMed

Proc Natl Acad Sci U S A. 1984 Jul;81(13):4188-92 PubMed

J Virol. 1986 Feb;57(2):422-32 PubMed

J Virol. 1986 Nov;60(2):589-98 PubMed

J Natl Cancer Inst. 1987 Aug;79(2):207-11 PubMed

Cell. 1987 Oct 23;51(2):211-20 PubMed

Folia Biol (Praha). 1987;33(4):253-7 PubMed

J Immunol Methods. 1987 Nov 5;103(2):247-52 PubMed

Proc Natl Acad Sci U S A. 1987 Oct;84(20):7041-5 PubMed

Virus Res. 1987 Aug;8(2):81-102 PubMed

Lancet. 1988 Jan 2-9;1(8575-6):5-9 PubMed

J Virol. 1988 Apr;62(4):1203-10 PubMed

Nature. 1970 Aug 15;227(5259):680-5 PubMed

Immunological quantification of RecA protein in cell extracts of E. coli after exposure to chemical mutagens or UV radiation