Increased synthesis of RecA protein is induced in E. coli cells after their damage, the rate of synthesis being dependent on the extent of DNA alterations. The level of the RecA protein was determined in E. coli cell extracts after damage induced by NQO, MNNG, MMC, NAL or UV radiation, using competitive enzyme-linked immunosorbent assay (ELISA). Purified E. coli RecA protein and rabbit monospecific polyclonal antibodies against it were prepared for the quantitative assay. The level of the RecA protein was increased after treatment with all mutagens. Contrary to other induced proteins, the synthesis of the RecA protein increased within 30 min after damage with UV radiation at a relatively slow rate. The ELISA method made it possible to determine 0.5-50 ng of the RecA protein in bacterial extracts. The method can be employed as an auxiliary test for DNA damage determination and also in studied concerning the role of the RecA protein in repair processes.

Cancer Research Institute Slovak Academy of Sciences Bratislava

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Biochimie. 1982 Aug-Sep;64(8-9):775-81 PubMed

Nature. 1970 Aug 15;227(5259):680-5 PubMed

Biochimie. 1984 Jun;66(6):471-6 PubMed

J Biol Chem. 1981 Jul 25;256(14):7557-64 PubMed

Biochimie. 1982 Apr;64(4):239-46 PubMed

Biochimie. 1985 Mar-Apr;67(3-4):349-52 PubMed

Proc Natl Acad Sci U S A. 1983 Jan;80(1):65-9 PubMed

Anal Biochem. 1985 Oct;150(1):228-34 PubMed

J Mol Biol. 1990 Mar 5;212(1):79-96 PubMed

J Mol Biol. 1975 Feb 25;92(2):341-56 PubMed

Microbiol Rev. 1984 Mar;48(1):60-93 PubMed

J Mol Biol. 1979 Jun 25;131(2):287-302 PubMed

Mol Gen Genet. 1982;185(2):275-82 PubMed

Anal Biochem. 1980 Mar 1;102(2):459-71 PubMed

J Biol Chem. 1981 May 10;256(9):4676-8 PubMed

Anal Biochem. 1976 May 7;72:248-54 PubMed

Proc Natl Acad Sci U S A. 1980 Sep;77(9):5375-9 PubMed

Mutat Res. 1985 Jun;147(3):65-78 PubMed

Br J Cancer. 1989 Oct;60(4):572-5 PubMed

J Bacteriol. 1981 Oct;148(1):386-90 PubMed

J Mol Biol. 1987 Apr 20;194(4):621-34 PubMed