An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.

Annotation

NIM; IMMUNOSORBENTS is also available

DUI

D004797 MeSH Browser

CUI

M0007526

Previous indexing

Immunoassay (1966-1976); Serodiagnosis (1966-1976)

History note

86(77); was see under IMMUNOENZYME TECHNIQUES 1977-85

Public note

86; was see under IMMUNOENZYME TECHNIQUES 1977-85