Peculiarities of amino acid transport in Schizosaccharomyces pombe: effects of growth medium
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články
PubMed
2606378
DOI
10.1007/bf02814469
Knihovny.cz E-zdroje
- MeSH
- aktivní transport MeSH
- aminokyseliny farmakokinetika MeSH
- cykloheximid farmakologie MeSH
- fungální proteiny biosyntéza MeSH
- kinetika MeSH
- kultivační média MeSH
- kyseliny aminoisomáselné farmakokinetika MeSH
- prolin farmakokinetika MeSH
- Saccharomycetales metabolismus MeSH
- Schizosaccharomyces účinky léků růst a vývoj metabolismus MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- 2-aminoisobutyric acid MeSH Prohlížeč
- aminokyseliny MeSH
- cykloheximid MeSH
- fungální proteiny MeSH
- kultivační média MeSH
- kyseliny aminoisomáselné MeSH
- prolin MeSH
Transport systems for amino acids in the wild-type strain of Schizosaccharomyces pombe are not constitutive. During growth on different media no transport of acidic, neutral and basic amino acids is detectable. To acquire the ability to transport amino acids, cells must be preincubated with a metabolic source of energy, such as glucose. The appearance of transport activity is associated with protein synthesis (suppression by cycloheximide) at all phases of culture growth. After such preincubation the initial rate of amino acid uptake depends on the phase of growth of the culture and on the amount of glucose in the growth medium but not on the nitrogen source used. L-Proline and 2-aminoisobutyric acid are practically not transported under any of the conditions tested.
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