Cloning of a thermostable alpha-amylase gene from Thermomonospora curvata and its expression in Streptomyces lividans
Language English Country England, Great Britain Media print
Document type Journal Article
- MeSH
- Actinomycetaceae enzymology MeSH
- alpha-Amylases biosynthesis genetics MeSH
- Genes, Bacterial MeSH
- DNA, Bacterial MeSH
- Electrophoresis, Polyacrylamide Gel MeSH
- Genes, Fungal MeSH
- Cloning, Molecular MeSH
- Gene Expression Regulation, Bacterial * MeSH
- Restriction Mapping MeSH
- Streptomyces enzymology genetics MeSH
- Hot Temperature MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- alpha-Amylases MeSH
- DNA, Bacterial MeSH
The gene from Thermomonospora curvata CCM 3312 coding for thermostable alpha-amylase (tam) has been cloned in Streptomyces lividans TK 24 and localized to a 2.6 kb HindIII-BamHI fragment of DNA. The data presented here show that the tam gene is expressed at a high level in S. lividans and that the protein is efficiently excreted.
Department of General Microbiology Institute of Microbiology Czechoslovak Academy of Sciences Prague
References provided by Crossref.org
Isolation of DNA-dependent RNA polymerase from the thermophilic actinomycete Thermomonospora curvata
