Concentration of retroviruses by low-speed centrifugation
Language English Country Slovakia Media print
Document type Journal Article
Persistent link
https://www.medvik.cz/link/pmid02851744
PubMed
2851744
Knihovny.cz E-resources
- MeSH
- Cell Line MeSH
- Centrifugation methods MeSH
- Electrophoresis, Polyacrylamide Gel MeSH
- Molecular Weight MeSH
- Retroviridae isolation & purification MeSH
- RNA, Viral biosynthesis isolation & purification MeSH
- Viral Proteins isolation & purification MeSH
- Mammary Tumor Virus, Mouse isolation & purification MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- RNA, Viral MeSH
- Viral Proteins MeSH
Concentration of retroviruses from volumes of up to 6 liters of medium by low-speed centrifugation is described. In contrast to pelleting, no damage or aggregation of particles was observed. Surface glycoproteins were also fully preserved. This method enables simple handling of relatively large volumes of medium. Highly purified mouse mammary tumor virus (MMTV) was obtained and its transframe protein p30 in SDS-PAGE was recognized as a double band.
