Concentration of retroviruses by low-speed centrifugation
Jazyk angličtina Země Slovensko Médium print
Typ dokumentu časopisecké články
Perzistentní odkaz
https://www.medvik.cz/link/pmid02851744
PubMed
2851744
Knihovny.cz E-zdroje
- MeSH
- buněčné linie MeSH
- centrifugace metody MeSH
- elektroforéza v polyakrylamidovém gelu MeSH
- molekulová hmotnost MeSH
- Retroviridae izolace a purifikace MeSH
- RNA virová biosyntéza izolace a purifikace MeSH
- virové proteiny izolace a purifikace MeSH
- virus myšího tumoru prsní žlázy izolace a purifikace MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- RNA virová MeSH
- virové proteiny MeSH
Concentration of retroviruses from volumes of up to 6 liters of medium by low-speed centrifugation is described. In contrast to pelleting, no damage or aggregation of particles was observed. Surface glycoproteins were also fully preserved. This method enables simple handling of relatively large volumes of medium. Highly purified mouse mammary tumor virus (MMTV) was obtained and its transframe protein p30 in SDS-PAGE was recognized as a double band.
