Isolation, characterization and utilization of the fifth component (C5) of porcine complement
Jazyk angličtina Země Česko Médium print
Typ dokumentu časopisecké články
PubMed
2943607
Knihovny.cz E-zdroje
- MeSH
- buněčná membrána imunologie MeSH
- inbrední kmeny myší MeSH
- kompetitivní vazba MeSH
- komplement C5 izolace a purifikace MeSH
- makrofágy imunologie MeSH
- myši MeSH
- prasata MeSH
- receptory Fc metabolismus MeSH
- receptory IgG MeSH
- receptory komplementu 3b MeSH
- receptory komplementu metabolismus MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- complement C5b receptor MeSH Prohlížeč
- komplement C5 MeSH
- receptory Fc MeSH
- receptory IgG MeSH
- receptory komplementu 3b MeSH
- receptory komplementu MeSH
The fifth component (C5) of porcine complement was isolated in immunochemically pure, homogeneous and native form. The isolated component was characterized, it has a molecular weight of 187,000 (under non-reducing conditions) and two non-identical chains of 112,000 and 76,000 under reducing conditions, respectively. The isolated C5 can be used for the detection of the C5b receptors on murine macrophages. Our findings are in good agreement with the results obtained using a rabbit and a human model, which suggests a high conservativeness of C5 during evolution. The described method of isolation of C5 from easily available porcine serum seems to be convenient for C5 receptors studies.
