Transfer of plasmids from Escherichia coli and Pseudomonas aeruginosa to methylotrophic bacteria and their detection in the new hosts
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články
PubMed
3121477
DOI
10.1007/bf02887565
Knihovny.cz E-zdroje
- MeSH
- Escherichia coli genetika MeSH
- Euryarchaeota genetika MeSH
- fenotyp MeSH
- genotyp MeSH
- konjugace genetická * MeSH
- plazmidy * MeSH
- Pseudomonas aeruginosa genetika MeSH
- Publikační typ
- časopisecké články MeSH
Several plasmids of incompatibility group P were transferred from Escherichia coli and Pseudomonas aeruginosa strains to Methylophilus methylotrophus and two other methylotrophs to test their recipient ability. The presence of plasmids in transconjugants was confirmed by electrophoretic analysis. Optimal conditions for detection of plasmid DNA in the strains tested based on alkaline lysis of cells at elevated temperature were established. Special behaviour of plasmids carrying the Mu phage in methylotrophic hosts is described.
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General and molecular microbiology and microbial genetics in the IM CAS
Mini-Mu transposition of bacterial genes on the transmissible plasmid
