The spleen colony technique. III. Comparison of the overlap effect and of errors in CFU-s determination and the [3H]-thymidine suicide data for several strains of mice
Jazyk angličtina Země Velká Británie, Anglie Médium print
Typ dokumentu časopisecké články
- MeSH
- analýza kolonii tvořících jednotek metody MeSH
- hematopoetické kmenové buňky cytologie MeSH
- inbrední kmeny myší MeSH
- interfáze MeSH
- myši MeSH
- slezina cytologie MeSH
- teoretické modely MeSH
- thymidin toxicita MeSH
- viabilita buněk účinky léků MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- thymidin MeSH
A mathematical model of errors of the spleen colony technique is applied to data obtained from four mouse strains and F1 hybrids. The variance of the colony counts was close to the Poisson distribution in inbred mice and F1 hybrids. However, it should be checked regularly. The magnitude of the error in CFU-s determination and of the estimations of the S phase fraction was derived, and is presented relative to the mean colony counts for all mouse strains studied. The optimum spleen colony counts are generally higher than those which are commonly used. However, the utilization of the optimum spleen colony counts requires a correction for the effect of colony overlap.
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