Identification of very-long-chain fatty acids in rat and mouse harderian gland lipids by capillary gas chromatography-mass spectrometry
Jazyk angličtina Země Nizozemsko Médium print
Typ dokumentu časopisecké články
- MeSH
- chromatografie na tenké vrstvě MeSH
- druhová specificita MeSH
- Harderova žláza analýza MeSH
- inbrední kmeny potkanů MeSH
- krysa rodu Rattus MeSH
- lipidy analýza MeSH
- mastné kyseliny analýza MeSH
- myši inbrední ICR MeSH
- myši MeSH
- plynová chromatografie s hmotnostně spektrometrickou detekcí MeSH
- slzné ústrojí analýza MeSH
- zvířata MeSH
- Check Tag
- krysa rodu Rattus MeSH
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- lipidy MeSH
- mastné kyseliny MeSH
Lipids of Harderian ophthalmic gland were separated by means of thin-layer chromatography with flame ionization detection in an latroscan apparatus. Wax ester and polar lipids (phosphatidylethanolamine and phosphatidylcholine) were detected as the main lipids in rats and glyceryl ether diester and both polar lipids were the main lipids in mice. Fatty acids were determined in individual lipid classes by means of gas chromatography and gas chromatography-mass spectrometry on capillary columns. The content of fatty acids, the positional isomers of monoenoic acids being predominantly C18, C20 and C22, is most interesting. Very-long-chain fatty acids, saturated fatty acids up to C30 and even monoenoic acids up to C28 were detected. Branched-chain fatty acids, predominantly iso and anteiso, are minority components, although their chain length distribution (C15-C27) is broad.
Citace poskytuje Crossref.org
