Cultivation on cellophane agar as a method for the assay of bacterial enzymes
Language English Country United States Media print
Document type Journal Article
PubMed
3286418
DOI
10.1007/bf02928080
Knihovny.cz E-resources
- MeSH
- Agar * MeSH
- alpha-Amylases biosynthesis MeSH
- Bacteria enzymology growth & development MeSH
- Bacteriological Techniques MeSH
- Cellophane * MeSH
- Culture Media * MeSH
- Metalloendopeptidases biosynthesis MeSH
- Thiosulfate Sulfurtransferase biosynthesis MeSH
- Urease biosynthesis MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Agar * MeSH
- alpha-Amylases MeSH
- Cellophane * MeSH
- Culture Media * MeSH
- Metalloendopeptidases MeSH
- neutral proteinase, cervical MeSH Browser
- Thiosulfate Sulfurtransferase MeSH
- Urease MeSH
Production of bacterial enzymes in a liquid medium and on cellophane-agar plates is compared. Using neutral proteinase, urease, alpha-amylase and thiosulfate sulfurtransferase as examples it was demonstrated that the cultivation on cellophane-agar plates yields higher concentrations of the enzymes and that the enzyme production is detected in a higher number of cultures. The method might be useful for the characterization of bacterial communities.
