A new way to carcinogenicity of azo dyes: the benzenediazonium ion formed from a non-aminoazo dye, 1-phenylazo-2-hydroxynaphthalene(Sudan I) by microsomal enzymes binds to deoxyguanosine residues of DNA
Language English Country Ireland Media print
Document type Journal Article
PubMed
3383184
DOI
10.1016/0304-3835(88)90092-4
PII: 0304-3835(88)90092-4
Knihovny.cz E-resources
- MeSH
- Coloring Agents * MeSH
- Biotransformation MeSH
- Deoxyguanosine metabolism MeSH
- Diazonium Compounds metabolism MeSH
- DNA metabolism MeSH
- Rats, Inbred Strains MeSH
- Microsomes, Liver metabolism MeSH
- Carcinogens * MeSH
- Rats MeSH
- Naphthols metabolism MeSH
- Chromatography, High Pressure Liquid MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Male MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- 1-phenylazo-2-naphthol MeSH Browser
- Coloring Agents * MeSH
- benzenediazonium MeSH Browser
- Deoxyguanosine MeSH
- Diazonium Compounds MeSH
- DNA MeSH
- Carcinogens * MeSH
- Naphthols MeSH
1-Phenylazo-2-hydroxynaphthalene (Sudan I) activated by pre-incubation with microsomal enzymes of rat livers covalently binds to DNA from calf thymus. Benzenediazonium ion formed from Sudan I by activation with microsomal enzymes is the principal active metabolite, which binds to DNA. Enzymatic hydrolysis of modified (14C-labelled) DNA, followed by separation of deoxynucleosides on a Sephadex G-10 column revealed that deoxyguanosine is the principal target for the binding of activated Sudan I. The high-performance liquid chromatographic (HPLC) analysis indicate that probably more than one radioactive adduct of activated Sudan I with deoxyguanosine is formed.
References provided by Crossref.org
