A new way to carcinogenicity of azo dyes: the benzenediazonium ion formed from a non-aminoazo dye, 1-phenylazo-2-hydroxynaphthalene(Sudan I) by microsomal enzymes binds to deoxyguanosine residues of DNA
Jazyk angličtina Země Irsko Médium print
Typ dokumentu časopisecké články
PubMed
3383184
DOI
10.1016/0304-3835(88)90092-4
PII: 0304-3835(88)90092-4
Knihovny.cz E-zdroje
- MeSH
- barvicí látky * MeSH
- biotransformace MeSH
- deoxyguanosin metabolismus MeSH
- diazoniové sloučeniny metabolismus MeSH
- DNA metabolismus MeSH
- inbrední kmeny potkanů MeSH
- jaterní mikrozomy metabolismus MeSH
- karcinogeny * MeSH
- krysa rodu Rattus MeSH
- naftoly metabolismus MeSH
- vysokoúčinná kapalinová chromatografie MeSH
- zvířata MeSH
- Check Tag
- krysa rodu Rattus MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- 1-phenylazo-2-naphthol MeSH Prohlížeč
- barvicí látky * MeSH
- benzenediazonium MeSH Prohlížeč
- deoxyguanosin MeSH
- diazoniové sloučeniny MeSH
- DNA MeSH
- karcinogeny * MeSH
- naftoly MeSH
1-Phenylazo-2-hydroxynaphthalene (Sudan I) activated by pre-incubation with microsomal enzymes of rat livers covalently binds to DNA from calf thymus. Benzenediazonium ion formed from Sudan I by activation with microsomal enzymes is the principal active metabolite, which binds to DNA. Enzymatic hydrolysis of modified (14C-labelled) DNA, followed by separation of deoxynucleosides on a Sephadex G-10 column revealed that deoxyguanosine is the principal target for the binding of activated Sudan I. The high-performance liquid chromatographic (HPLC) analysis indicate that probably more than one radioactive adduct of activated Sudan I with deoxyguanosine is formed.
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