Preparation and characterization of a monoclonal antibody against boar acrosin
Folia biologica.
1986 ;
32 (4) :
282-5.
Language English Country Czech Republic Media print
Document type Journal Article
Persistent link
https://www.medvik.cz/link/pmid03530827
PubMed
3530827
Knihovny.cz E-resources
- MeSH
- Acrosin immunology isolation & purification MeSH
- Endopeptidases immunology MeSH
- Antibodies, Monoclonal * isolation & purification MeSH
- Mice, Inbred BALB C MeSH
- Mice MeSH
- Swine MeSH
- Spermatozoa enzymology MeSH
- Animals MeSH
- Check Tag
- Male MeSH
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Acrosin MeSH
- Endopeptidases MeSH
- Antibodies, Monoclonal * MeSH
BALB/c mice were immunized with the crude fraction of boar acrosin. Immune spleen cells were fused with myeloma cells SP2/0-Ag14. One of the 6 hybridomas produced was cloned and characterized by ELISA and SDS-PAGE. Possible uses of the monoclonal antibody against acrosin for immunological detection of the amount of acrosin liberated after manipulations with spermatozoa and for selection of undamaged spermatozoa for insemination are discussed.
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2017 May ;
77 (7) :
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