Interaction of bilirubin with human lymphocytes and granulocytes--the effect on bilirubin metabolism
Language English Country United States Media print
Document type Journal Article
PubMed
3792964
DOI
10.1007/bf02936604
Knihovny.cz E-resources
- MeSH
- Bilirubin metabolism MeSH
- Granulocytes drug effects immunology MeSH
- Potassium Cyanide pharmacology MeSH
- Humans MeSH
- Lymphocytes drug effects immunology MeSH
- Serum Albumin, Bovine metabolism MeSH
- Temperature MeSH
- Ultrasonics MeSH
- Check Tag
- Humans MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Bilirubin MeSH
- Potassium Cyanide MeSH
- Serum Albumin, Bovine MeSH
An in vitro degradation of bilirubin by isolated human granulocytes and lymphocytes is described. Changes in the bilirubin concentration during interaction with these cells were determined spectrophotometrically. The dependence of the reaction velocity on the original bilirubin concentration followed an adsorption isotherm, typical of enzyme processes with a Km of 79 microM at a cell concentration of 500/microL. The primary event is the adsorption of bilirubin to the cell surface and, in addition, its detoxication. Cyanide and sonic treatment of cells inhibit the reaction, salicylic acid enhances the cell activity. The bilirubin-detoxicating effect is discussed with respect to the therapy of hyperbilirubinaemia in neonates.
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