Purification of streptococcal extracellular NAD+ nucleosidase is associated with changes of kinetic properties. A high-molecular weight component is required for a full activity of the enzyme. The component is not produced by bacteria and is present in the Todd-Hewitt cultivation medium, the beef-heart extract serving primarily as its source. In its effect on the enzyme this component can be replaced with bovine albumin. It follows from the time relationship of the NAD decomposition that the mechanism of the studied enzymic reaction is not hydrolytic. In addition to nicotinamide, a product of polymeric character is formed.

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J Exp Med. 1956 Oct 1;104(4):577-87 PubMed

J Biol Chem. 1967 Jul 10;242(13):3164-71 PubMed

J Hyg Epidemiol Microbiol Immunol. 1970;14(1):123-4 PubMed

J Biol Chem. 1969 Apr 10;244(7):1838-45 PubMed

J Biol Chem. 1970 Apr 10;245(7):1626-31 PubMed

Vestn Akad Med Nauk SSSR. 1966;21(4):3-9 PubMed

J Biol Chem. 1967 Jul 10;242(13):3172-9 PubMed

Arch Biochem Biophys. 1974 Feb;160(2):530-51 PubMed

Biochemistry. 1968 Nov;7(11):3979-90 PubMed

J Biol Chem. 1969 Apr 10;244(7):1831-7 PubMed

Cancer Res. 1969 Aug;29(8):1568-73 PubMed

Biochim Biophys Acta. 1967 Sep 26;145(2):247-59 PubMed

Anal Biochem. 1968 Oct 24;25(1):99-108 PubMed

Folia Microbiol (Praha). 1977;22(2):92-7 PubMed

Eur J Biochem. 1974 Dec 16;50(1):145-55 PubMed

Eur J Biochem. 1975 Mar 3;52(1):117-23 PubMed

J Biol Chem. 1958 Apr;231(2):727-40 PubMed

FEBS Lett. 1975 May 15;53(3):274-8 PubMed

J Exp Med. 1957 Jul 1;106(1):15-26 PubMed

Adv Enzyme Regul. 1969;7:397-413 PubMed

Biochim Biophys Acta. 1967 Apr 18;138(2):438-41 PubMed

Biochem J. 1964 Feb;90(2):284-92 PubMed

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