Degradation of continuously added 3-chlorobenzoate (3-CB) was studied in samples of chernozem soil. Soil columns were inoculated with Pseudomonas putida growing on 3-CB and carrying the biodegradation plasmid and with Pseudomonas aeruginosa incapable of growth on 3-CB and carrying the inserted biodegradation plasmid pBS 2 determining ortho-cleavage of the aromatic ring. While the 3-CB degradation was observed in both inoculated variants, the native microflora of the soil under study was incapable to degrade 3-CB. Among pseudomonads isolated from inoculated soil at different stages of cultivation and growth on 3-CB, some had the taxonomic features of P. putida as well as those differing in 1-5 characteristics. The study of the activities of the enzymes cleaving the aromatic ring revealed the presence of pyrocatechol 1,2-dioxygenase in the isolated strains only, as estimated by means of benzoate and 3-CB as substrates.

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Mikrobiologiia. 1983 Sep-Oct;52(5):771-6 PubMed

Mikrobiologiia. 1982 Mar-Apr;51(2):275-80 PubMed

Mikrobiologiia. 1982 Nov-Dec;51(6):973-8 PubMed

Appl Environ Microbiol. 1982 Aug;44(2):514-6 PubMed

Degradation of 2-chlorobenzoic and 2,5-dichlorobenzoic acids in soil columns by Pseudomonas stutzeri