Mutants of Aspergillus niger 194A and 178 requiring lysine differ from the original prototrophic strain K10 and from each other on the course of accumulation of organic acids. In both mutants less citric acid accumulates during the first phase of cultivation but considerably more 2-oxoglutarate and 2-oxoadipate accumulate than in the original strain. Whereas in the 194A mutant this state remains unchanged also during the second phase of cultivation, in the 178 mutant oxo acids are degraded and citric acid is synthesized intensively. The accumulation of 2-oxoglutarate and 2-oxoadipate in the fermentation medium indicates that in A. niger lysine is synthesized via the homocitrate pathway.

See more in PubMed

Appl Microbiol. 1961 Jan;9(1):1-5 PubMed

Genetics. 1971 Mar;67(3):365-76 PubMed

Z Allg Mikrobiol. 1977;17(1):81-3 PubMed

Arch Biochem Biophys. 1968 Feb;123(2):235-9 PubMed

Arch Biochem Biophys. 1955 Mar;55(1):270-3 PubMed

J Biol Chem. 1968 Apr 25;243(8):1839-45 PubMed

Folia Microbiol (Praha). 1970;15(1):35-9 PubMed

J Biol Chem. 1969 Mar 25;244(6):1417-23 PubMed

Biochem Biophys Res Commun. 1964;14:262-7 PubMed

J Biol Chem. 1965 Nov;240(11):4357-61 PubMed

Annu Rev Biochem. 1968;37:79-108 PubMed

J Biol Chem. 1967 May 25;242(10):2542-6 PubMed

J Bacteriol. 1967 Oct;94(4):1060-5 PubMed

Biochem Biophys Res Commun. 1964;14:268-71 PubMed