Nystatin-resistant yeast Candida tropicalis was obtained after UV illumination and plating on nystatin-containing media. The mutant contained no ergosterol in the plasma membrane but bound nystatin to a degree similar to that of the wild strain (1.2 vs. 1.5 nmol per mg dry solid). Respiration of the mutant on glucose was reduced by 36% in the presence of 25 microM nystatin. This corresponded to a 25-43% decrease of the uptake of monosaccharides. Transport of amino acids was reduced by nystatin in the mutant by 44-86%, as compared with a 84-95% reduction in the wild strain. The intracellular ATP content was reduced by nystatin equally in the wild strain and in the mutant (by 43 and 47%). Nystatin appears to affect specifically membrane transport processes of nonelectrolytes while both the H+-extruding ATPase and the membrane potential are unaffected.

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Microbios. 1981;30(121-122):139-51 PubMed

FEBS Lett. 1978 Jul 15;91(2):198-201 PubMed

Z Klin Chem Klin Biochem. 1967 Nov;5(6):299-304 PubMed

Biochim Biophys Acta. 1971 Aug 13;241(2):310-30 PubMed

Biochim Biophys Acta. 1981 Apr 22;643(1):265-8 PubMed

Biochim Biophys Acta. 1981 Dec 30;639(3-4):197-223 PubMed

Microbios. 1981;31(123):7-16 PubMed

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