Valine production from hydrocarbon by Micrococcus varians
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články
PubMed
7141324
DOI
10.1007/bf02877121
Knihovny.cz E-zdroje
- MeSH
- antibakteriální látky farmakologie MeSH
- kultivační média MeSH
- Micrococcus účinky léků růst a vývoj metabolismus MeSH
- polysorbáty farmakologie MeSH
- uhlovodíky metabolismus MeSH
- valin analýza biosyntéza MeSH
- vitaminy farmakologie MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- antibakteriální látky MeSH
- kultivační média MeSH
- polysorbáty MeSH
- uhlovodíky MeSH
- valin MeSH
- vitaminy MeSH
A bacterium isolated from Assam (India) soil was found to accumulate L-valine in the growth medium and was identified as Micrococcus varians. The strain grew and accumulated valine in a purely synthetic medium, but supplementation with either casamino acids or yeast extract or with both, improved the yield. The entire fermentation period could be divided into a growth phase and a production (phase which could be prolonged by adjustment of pH to neutral range. Among the different hydrocarbon and nitrogen sources tested straight run gas-oil and ammonium sulphate, respectively, were found most suitable. Antibiotics inhibited growth but stimulated extracellular valine accumulation. Vitamins stimulated growth and valine yield and an inoculum level of 10% was found to be optimal. The yield of L-valine under optimal conditions was 2.95 g/L.
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