Beryllium toxicity testing in the suspension culture of mouse fibroblasts
Jazyk angličtina Země Česko Médium print
Typ dokumentu časopisecké články
PubMed
7190987
Knihovny.cz E-zdroje
- MeSH
- beryllium farmakologie toxicita MeSH
- buněčné dělení účinky léků MeSH
- buněčné linie MeSH
- fibroblasty MeSH
- myši MeSH
- síran hořečnatý farmakologie MeSH
- vztah mezi dávkou a účinkem léčiva MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- beryllium MeSH
- síran hořečnatý MeSH
Suspension culture of mouse fibroblast cell line L-A 115 was used to test beryllium toxicity in the presence of magnesium ions. Beryllium added to the MEM cultivation medium was bound in a complex with sulphosalicylic acid BeSSA complex, because the use of beryllium chloride turned out to yield ineffective beryllium phosphate that formed macroscopically detectable insoluble opacities. The BeSSA complex was used in the concentration range: 10(-3)--10(-9)M, magnesium was used in 3 concentrations: 10(-1)M, 5 x 10(-2)M and 10(-2)M. Growth curve analysis revealed pronounced beryllium toxicity at the concentration of 10(-3)M, magnesium-produced toxic changes were observed only at the concentration of 10(-1)M. No competition between the beryllium and magnesium ions was recorded. It is assumed that the possible beryllium-magnesium competition was significantly modified by the use of BeSSA complex-bound beryllium.