Enhanced chemiluminescent sandwich enzyme immunoassay for hen egg lysozyme
Language English Country England, Great Britain Media print
Document type Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
PubMed
7762414
DOI
10.1002/bio.1170100106
Knihovny.cz E-resources
- MeSH
- Food Analysis MeSH
- Immunoenzyme Techniques MeSH
- Indicators and Reagents MeSH
- Colorimetry methods MeSH
- Chickens MeSH
- Luminescent Measurements MeSH
- Muramidase analysis MeSH
- Sensitivity and Specificity MeSH
- Eggs * MeSH
- Animals MeSH
- Check Tag
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Comparative Study MeSH
- Names of Substances
- Indicators and Reagents MeSH
- Muramidase MeSH
A sensitive chemiluminescent sandwich-type enzyme immunoassay for hen egg lysozyme was developed. The assay was performed on polystyrene microtitre plates using immobilized specific polyclonal rabbit antibody against lysozyme, a peroxidase conjugate and the H2O2/luminol-enhanced chemiluminescence detection reagent. The chemiluminescent signal was detected using either a microplate luminometer, or photographic film in a camera luminometer. The detection limit for lysozyme was 0.3 ng/mL, and this was three times lower than that obtained using a colorimetric method with H2O2 and o-phenylendiamine as substrates. Recovery of the assay was 97-112% and the relative standard deviation ranged from 3.6% to 10.3%. The immunoassay overcame interference from the food sample matrix when lysozyme, used as a bacteriostatic agent, was measured.
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