Pectinolytic enzymes of anaerobic fungi
Jazyk angličtina Země Velká Británie, Anglie Médium print
Typ dokumentu časopisecké články
- MeSH
- bachor mikrobiologie MeSH
- houby enzymologie MeSH
- koncentrace vodíkových iontů MeSH
- ovce MeSH
- polygalakturonasa metabolismus MeSH
- polysacharid-lyasy metabolismus MeSH
- velbloudi MeSH
- vysoká zvěř MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- pectate lyase MeSH Prohlížeč
- pectin lyase MeSH Prohlížeč
- polygalakturonasa MeSH
- polysacharid-lyasy MeSH
Pectinolytic enzymes of four rumen fungi have been described. Three fungal species were monocentric Neocallimastix spp. H15, JL3 and OC2, and one isolate was a polycentric strain of Orpinomyces joyonii, A4. They differed in degree of pectin degradation and utilization. Only the strain Neocallimastix sp. H15 and partially Orpinomyces joyonii A4 were able to utilize pectin to a higher extent. The most important pectinolytic activity in all these isolates represented pectin lyase (EC 4.2.2.10) and polygalacturonase (EC 3.2.1.15). Their specific activities were in the range of 100-900 and 10-450 micrograms galacturonic acid h-1 mg protein-1 for pectin lyase and polygalacturonase, respectively. Polygalacturonase, located mainly in the endocellular fraction, was inhibited by calcium ions and had the main pH optimum at pH 6.0. All strains produced pectate lyase (EC 4.2.2.2). None of the strains tested produced pectinesterase (EC 3.1.1.11).
Citace poskytuje Crossref.org
Isolation and characterization of rabbit caecal pectinolytic bacteria
