Protein kinase C in nucleus parabrachialis: effect of drugs inducing conditioned taste aversion
Jazyk angličtina Země Nizozemsko Médium print
Typ dokumentu časopisecké články
PubMed
8111635
DOI
10.1016/0006-8993(93)91340-x
PII: 0006-8993(93)91340-X
Knihovny.cz E-zdroje
- MeSH
- chlorid lithný farmakologie MeSH
- chuť účinky léků MeSH
- cytosol účinky léků metabolismus MeSH
- krysa rodu Rattus MeSH
- měď farmakologie MeSH
- operantní podmiňování účinky léků fyziologie MeSH
- pons účinky léků enzymologie fyziologie MeSH
- proteinkinasa C metabolismus MeSH
- sacharin farmakologie MeSH
- síran měďnatý MeSH
- učení vyhýbat se fyziologie MeSH
- zrakové korové centrum účinky léků metabolismus MeSH
- zvířata MeSH
- Check Tag
- krysa rodu Rattus MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- chlorid lithný MeSH
- měď MeSH
- proteinkinasa C MeSH
- sacharin MeSH
- síran měďnatý MeSH
A method for the dissection of the nucleus parabrachialis (NPB) from the coronal sections of frozen rat brain is described. The protein kinase C (PKC) activity was determined in the cytosol and particulate fractions of the pooled samples of the nucleus. The effect of the i.p. administration of the conditioned taste aversion-inducing agents LiCl and CuSO4 on PKC in the NPB and visual cortex (VC) was tested. 1 h after the LiCl injection, the portion of the membrane-bound PKC was increased by 23% (P < 0.01) above the level found after the saline injection. CuSO4 produced a 19% increase. Since the PKC activity in the cytosol declined, it is likely that the translocation of the enzyme took place. No changes in the PKC distribution in the VC samples could be detected. The results support the idea that the PKC translocation is not directly induced by the tested substances but that it rather reflects changes in the activity of the visceral system.
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